Feedback inhibition of actin on Rho mediates content release from large secretory vesicles.
Feedback inhibition of actin on Rho mediates content release from large secretory vesicles.
复制标题
肌动蛋白对 Rho 的反馈抑制介导大分泌囊泡内容物的释放。
DOI：
10.1083/jcb.201711006
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Shilo,Ben-Zion
中科院分区：
文献类型：
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作者：
Segal,Dagan;Zaritsky,Assaf;Schejter,EyalD;Shilo,Ben-Zion
收缩的光化素结构的协调形成和拆解已被证明是组织形态发生的不同背景的基础。这些事件不受基因表达的直接调控，而是依赖于固有的机械性特征以及对紧张和压力的反应(Guillot和Lecuit，2013；Pandya等人，2017)。肌球蛋白动力学在时间和空间上的严密调控调节了这种细胞收缩事件的级联，以及同一基本机械在不同生物场景中的多功能活动，仍有待完全了解。果蝇幼虫唾液腺上皮的排泄为研究肌球蛋白在收缩事件中的协调调控提供了一个强大的系统(Tran and Ten Hagen，2017)。在激素提示的刺激下，这些细胞在2小时内从它们的顶膜分泌大量的“胶状”糖蛋白进入腺腔，为外部沉积做准备，使幼虫在羽化前能够附着在干燥的表面上(Biyasheva等人，2001年)。胶蛋白在分泌前储存在非常大的囊泡中，直径从4到8微米不等。这些小泡与细胞顶膜融合后，通过在每个小泡周围形成一层肌动蛋白涂层和肌球蛋白的募集，实现了内容物从这些小泡到管腔的释放，这两个因素共同调节了这种力量。
Coordinated formation and disassembly of contractile actinbased structures has been shown to underlie diverse settings of tissue morphogenesis. These events are not regulated directly by gene expression, but rather rely on inherent mechanistic features, as well as responses to tension and stress (Guillot and Lecuit, 2013; Pandya et al., 2017). The tight temporal and spatial regulation of actomyosin dynamics mediating such cascades of cellular contractile events, and the versatile activity of the same basic machinery in diverse biological scenarios, remain to be fully understood.Exocytosis from the epithelium of Drosophila melanogaster larval salivary glands provides a powerful system to study the orchestrated regulation of actomyosin during contractile events (Tran and Ten Hagen, 2017). Upon stimulation by hormonal cues, these cells secrete large amounts of “glue” glycoproteins from their apical membrane into the lumen of the gland over the course of 2 h, in preparation for external deposition that will enable the larva to adhere to a dry surface before pupariation (Biyasheva et al., 2001). The glue proteins are stored before secretion in very large vesicles, ranging in size from 4 to 8 µm in diameter. Content release from these vesicles into the lumen is achieved after their fusion with the apical membrane of the cell, through formation of an actin coat around each vesicle and recruitment of myosin, which together mediate the forces