A highly selective DNAzyme sensor for mercuric ions
A highly selective DNAzyme sensor for mercuric ions
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DOI：
10.1002/anie.200800960
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发表时间：
2008-01-01
影响因子：
16.6
通讯作者：
Perrin, David M.
中科院分区：
文献类型：
--
作者：
Hollenstein, Marcel;Hipolito, Christopher;Perrin, David M.
材料：根据文献程序[1]合成dAimTP，dUaaTP购自Sigma-Aldrich。所有寡核苷酸均购自Integrated DNA Technologies（科拉尔维尔，IA），并通过10- 15%8 M尿素变性PAGE纯化。超纯dNTP获自Fermentas，而Sequenase Version 2.0和单链DNA结合蛋白购自GE Healthcare。λ核酸外切酶、Taq DNA聚合酶和Vent exo（-）DNA聚合酶获自新英格兰Biolabs。链霉亲和素磁性颗粒购自Roche。核苷三磷酸dGTP α-[32 P]购自Perkin Elmer。pGEM-T-Easy Vector Systems试剂盒获自Promega。LiClO 4购自JT Baker。除从Fisher购买的FeSO 4·7 H2O、Fe（NO3）3·9 H2O、Pb（NO3）2、UO 2（OAM）2·2 H2O、CoCl 2·7 H2O和CuSO 4外，Hg（OAM）2以及所有缓冲液和金属盐均从奥德里奇获得。
Materials: dAimTP was synthesized according to a literature procedure [1] and dUaaTP was purchased from Sigma-Aldrich. All oligonucleotides were purchased from Integrated DNA Technologies (Coralville, IA) and were purified by 10-15% 8 M urea denaturing PAGE. Ultrapure dNTPs were obtained from Fermentas, while Sequenase Version 2.0 and Single Stranded DNA binding protein were purchased from GE Healthcare. Lambda exonuclease, Taq DNA polymerase and Vent exo (-) DNA polymerase were obtained from New England Biolabs. Streptavidin magnetic particles were purchased from Roche. The nucleoside triphosphate dGTP α-[32P] was purchased from Perkin Elmer. pGEM-T-Easy Vector Systems kit was obtained from Promega. LiClO4 was purchased from JT Baker. Hg (OAc) 2 and all buffer and metal salts were obtained from Aldrich, save for FeSO4· 7H2O, Fe (NO3) 3· 9H2O, Pb (NO3) 2, UO2 (OAc) 2· 2H2O, CoCl2· 7H2O and CuSO4 which were purchased from Fisher.