Proteome Analysis of Differential Protein Expression in Brain of Rats with Type 1 Diabetes Mellitus
Proteome Analysis of Differential Protein Expression in Brain of Rats with Type 1 Diabetes Mellitus
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DOI：
10.1055/s-0031-1271705
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发表时间：
2011-05-01
影响因子：
1.8
通讯作者：
Jiang, Z. H.
中科院分区：
文献类型：
--
作者：
Li, X.;Pan, W.;Jiang, Z. H.
研究目的：应用蛋白质组学方法，对1型糖尿病（T1 DM）大鼠脑组织中差异表达的蛋白质进行鉴定，以寻找T1 DM脑组织病理改变的潜在生物标志物。从T1 DM大鼠和健康对照大鼠的脑组织中提取蛋白质，经双向凝胶电泳（2-DE）分离，基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）鉴定，Western blot分析和免疫组化（IHC）验证结果。利用MALDI-TOF-MS技术从24个差异表达点中鉴定出8个蛋白质，分别为维生素D依赖性钙结合蛋白、肌酸激酶B型（B-CK）、肌球蛋白轻链激酶（MLCK）、热休克蛋白60和71、ATP合成酶、细胞周期蛋白G、泛酸激酶1（PANK 1）。T1 DM组有3个蛋白表达上调，5个蛋白表达下调。8个蛋白质鉴定，MLCK被证实的Western blot和IHC。结论：这项工作表明，一个全面的蛋白质组学鉴定的策略应该是一个有用的工具，了解糖尿病脑病的机制。MLCK等差异蛋白可能为糖尿病脑病的发病机制提供线索。
Aim of the study: Proteomic approach was applied to identify differential protein expressed in brain of rats with type 1 diabetes mellitus (T1DM) in order to search for potential biomarkers for pathological changes of brain with T1DM.Methods: Proteins were extracted from brain tissues of T1DM rats and healthy control rats, separated by 2-DE and identified by MALDI-TOF-MS. The results were validated by Western blot analysis and immunohistochemistry (IHC).Results: A total of 8 proteins from the 24 differentially expressed spots were identified by MALDI-TOF-MS. The proteins identified were vitamin D-dependent calcium-binding protein, creatine kinase B-type (B-CK), myosin light chain kinase (MLCK), HSP60 and HSP71, ATP synthase, cyclin-G, pantothenate kinase-1 (PANK1), respectively. 3 proteins were up-regulated and 5 proteins were down-regulated from the T1DM rats. Of the 8 proteins identified, MLCK was confirmed by Western blot and IHC.Conclusion: This work demonstrates that a comprehensive strategy of proteomic identification should be a useful tool for understanding of diabetic encephalopathy mechanism. And the differential proteins such as MLCK may be give clues about the pathogenesis of diabetic encephalopathy.