Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies
Improved hepatocyte in vitro maintenance in a culture model with woven multicompartment capillary systems: Electron microscopy studies
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在具有编织多室毛细管系统的培养模型中改善肝细胞的体外维持：电子显微镜研究
DOI：
10.1002/hep.1840220226
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发表时间：
1995
期刊：
影响因子：
13.5
通讯作者：
Peter Neuhaus
中科院分区：
文献类型：
--
作者：
J. Gerlach;N. Schnoy;J. Encke;Mark D. Smith;C. Müller;Peter Neuhaus
猪原代肝细胞在体外培养模型中形成组织样结构，具有三维细胞定向、细胞聚集、低代谢梯度分散细胞灌注、整体氧合和非实质细胞共培养的功能。扫描电子显微镜（SEM）显示，肝细胞自发地在人造毛细血管之间和表面形成三维结构的聚集体。透射电镜（TEM）显示，体外灌注7周后，细胞超微结构仍与体内实质相似。高尔基复合物、活跃的膜过程、细胞连接的重组和胆管样的细胞间隙被证实。（国际肝病1995；22:546-552。）
Primary pig hepatocytes form a tissuelike structure in an in vitro culture model that has provision for threedimensional cell orientation, cell aggregation, decentralized cell perfusion with low metabolite gradients, integral oxygenation, and nonparenchymal cell coculture. Scanning electron microscopy (SEM) has shown that hepatocytes spontaneously form aggregates in a three‐dimensional structure between and on the surface of artificial capillaries. Transmission electron microscopy (TEM) has shown that after 7 weeks of in vitro perfusion, the cell ultrastructure remains similar to that of the parenchyma in vivo. Golgi complexes, active membrane processes, reorganization of cell junctions, and bile canaliculi‐like intercellular spaces were demonstrated.(Hepatology 1995; 22:546–552.)
DOI：
10.1073/pnas.85.7.2161
发表时间：
1988-04-01
影响因子：
11.1
作者：
BENZEEV, A;ROBINSON, GS;FARMER, SR
通讯作者：
FARMER, SR
有证据表明库普弗细胞的激活会增加冷藏后的摄氧量。
影响因子：
6.2
作者：
Fusaoka,T;Hunt,KJ;Lemasters,JJ;Thurman,RG
通讯作者：
Thurman,RG