DNA-templated gold nanoparticles formation.
DNA-templated gold nanoparticles formation.
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DNA 模板金纳米颗粒的形成。
DOI：
10.1166/jnn.2008.270
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发表时间：
2008
影响因子：
--
通讯作者：
Zhuang Li
中科院分区：
文献类型：
--
作者：
Lanlan Sun;Yonghai Song;Li Wang;Yujing Sun;Cun;Zhelin Liu;Zhuang Li
四氯金酸（HAuCl₄）与DNA之间的相互作用使得在不形成大纳米粒子的情况下能够制备出以DNA为模板的金纳米粒子。研究发现，通过改变反应条件，包括DNA浓度和反应温度，可以获得直径为8.7 nm的球形DNA - HAuCl₄杂化物、花状DNA - HAuCl₄杂化物、DNA - HAuCl₄杂化物的纳米粒子链以及纳米粒子网络。通过缩短DNA和HAuCl₄的反应时间对中间产物进行了研究，所获得的纳米粒子保留了一小段DNA片段，这表明DNA和HAuCl₄之间的反应是一个过程。分别加入还原剂后得到了以DNA为模板的金纳米粒子和纳米花。利用紫外 - 可见吸收光谱对DNA - HAuCl₄杂化物以及以DNA为模板的金纳米结构进行了表征，利用X射线光电子能谱（XPS）对DNA - Au(Ⅲ)配合物和金纳米粒子的组成进行了比较。原子力显微镜（AFM）和透射电子显微镜（TEM）结果显示，加入硼氢化钠（NaBH₄）后形成了尺寸约为11 nm的球形金纳米粒子和花状金纳米粒子。
The interaction between HAuCl4 and DNA has enabled creation of DNA-templated gold nanoparticles without formation of large nanoparticles. It was found that spheral DNA-HAuCl4 hybrid of 8.7 nm in diameter, flower-like DNA-HAuCl4 hybrid, nanoparticles chains and nanoparticles network of DNA-HAuCl4 hybrid could be obtained by varying the reaction conditions, including DNA concentration and reaction temperature. The intermediate product was investigated by shortening the reaction time of DNA and HAuCl4, and the obtained nanoparticles preserved a small DNA segment, which indicated that the reaction between DNA and HAuCl4 had a process. The addition of reduction reagent resulted in DNA-templated gold nanoparticles and nanoflowers, respectively. UV-vis absorption spectra were used to characterize the DNA-HAuCl4 hybrid and the gold nanostructures templated on DNA, and XPS spectra were used to compare the composition of DNA-Au(III) complex and gold nanoparticles. AFM and TEM results revealed that the spheral gold nanoparticles of about 11 nm in size and flower-like gold nanoparticles were formed after the addition of NaBH4.