Differential gene expression in well-regulated and dysregulated pancreatic β-cell (MIN6) sublines
Differential gene expression in well-regulated and dysregulated pancreatic β-cell (MIN6) sublines
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DOI：
10.1210/en.2002-220916
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发表时间：
2003-04-01
期刊：
影响因子：
4.8
通讯作者：
Irminger, JC
中科院分区：
文献类型：
--
作者：
Lilla, V;Webb, G;Irminger, JC
为了鉴定参与调节胰岛素分泌的基因，我们已经建立并表征了来自小鼠胰腺β细胞系MIN 6的两个亚系，命名为B1和C3。它们的胰岛素含量相似，但分泌特性不同。B1以浓度和细胞融合依赖的方式对葡萄糖作出反应，而C3则没有。B1细胞受到佛波醇12-肉豆蔻酸酯13-乙酸酯、亮氨酸、精氨酸、格列本脲、异丁基甲基黄嘌呤和KCl的刺激，而C3没有反应（亮氨酸、精氨酸和格列本脲）或反应程度较低（异丁基甲基黄嘌呤、佛波醇12-肉豆蔻酸酯13-乙酸酯和KCl）。虽然细胞内Ca 2+上升响应于葡萄糖在B1，但不是C3细胞，KCl增加细胞内Ca 2+在两个亚系中以类似的方式。B1和C3细胞中GLUT-1、GLUT-2、Kir6.2和SUR 1的表达没有显著差异，而E-cadherin在B1细胞中表达更丰富。通过抑制性消减杂交和高密度寡核苷酸芯片技术，建立了一个更完整的差异表达基因列表。根据已知或推定的功能将基因聚类。参与代谢、细胞内信号传导、细胞结构和细胞粘附的那些是潜在的兴趣。这两个亚系对于鉴定参与调节胰腺β细胞的胰岛素分泌的基因和机制是有用的。
To identify genes involved in regulated insulin secretion, we have established and characterized two sublines derived from the mouse pancreatic beta-cell line MIN6, designated B1 and C3. They have a similar insulin content, but differ in their secretory properties. B1 responded to glucose in a concentration- and cell confluence-dependent manner, whereas C3 did not. B1 cells were stimulated by phorbol 12-myristate 13-acetate, leucine, arginine, glibenclamide, isobutylmethylxanthine, and KCl, whereas C3 did not respond ( leucine, arginine, and glibenclamide) or responded to a lesser extent ( isobutylmethylxanthine, phorbol 12-myristate 13-acetate, and KCl). Although intracellular Ca2+ rose in response to glucose in B1 but not C3 cells, KCl increased intracellular Ca2+ in a similar manner in both sublines. GLUT-1, GLUT-2, Kir6.2, and SUR1 expression was not significantly different between B1 and C3 cells, whereas E-cadherin was more abundantly expressed in B1 cells. A more complete list of differentially expressed genes was established by suppression subtractive hybridization and high density (Affymetrix) oligonucleotide microarrays. Genes were clustered according to known or putative function. Those involved in metabolism, intracellular signaling, cytoarchitecture, and cell adhesion are of potential interest. These two sublines should be useful for identification of the genes and mechanisms involved in regulated insulin secretion of the pancreatic beta-cell.