MONOCLONAL M6 ANTIBODY INTERFERES WITH NEURITE EXTENSION OF CULTURED NEURONS
MONOCLONAL M6 ANTIBODY INTERFERES WITH NEURITE EXTENSION OF CULTURED NEURONS
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DOI：
10.1002/neu.480230108
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发表时间：
1992-02-01
期刊：
影响因子：
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通讯作者：
SCHACHNER, M
中科院分区：
文献类型：
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作者：
LAGENAUR, C;KUNEMUND, V;SCHACHNER, M
单克隆M6抗体与鼠中枢神经系统神经元的表面以及脉络丛和肾脏近端小管中上皮细胞的顶面结合。M6抗原早在胚胎第10天就在中枢神经系统中表达，在神经管的边缘区表达最强，并且在成年后仍然可以检测到。M6抗体的IgG或Fab片段干扰培养的小脑神经元的突起延伸。抗体对神经突延伸的作用在24小时后可容易地检测到。在抗体处理的前3天期间未检测到细胞活力的降低。在抗体存在下维持超过5天的培养物表现出降低的神经元活力。当培养基中含有25 mM KCl时，可在很大程度上避免存在M6抗体时长期活力的降低。抗体介导的神经突生长的扰动不被高KCl的存在所阻断。在这些培养物中，神经突的异常短和扁平的外观表明M6抗体选择性地影响神经突延伸。抗M6处理的神经元的延时摄影显示对生长锥的板状伪足和丝状伪足的运动没有明显的影响。只有神经突的整体延伸似乎受到抑制。M6抗原是一种35 kD的糖蛋白，可以从成年小鼠脑的脱氧胆酸盐（DOC）溶解的膜组分中分离出来。
Monoclonal M6 antibody binds to the surface of murine central nervous system neurons as well as to apical surfaces of epithelial cells in the choroid plexus and proximal tubules of the kidney. M6 antigen is expressed in the central nervous system as early as embryonic day 10, most strongly in the marginal zone of the neural tube, and remains detectable in adulthood. IgG or Fab fragments of M6 antibody interfere with the extension of neurites by cultured cerebellar neurons. Effects of the antibody on neurite extension are readily detectable after 24 h. No reduction of cell viability is detected during the first 3 days of antibody treatment. Cultures maintained in the presence of antibody for longer than 5 days exhibit reduced viability of neurons. This reduction in long-term viability in the presence of M6 antibody is largely avoided when 25 mM KCl is included in the culture medium. The antibody-mediated perturbation of neurite outgrowth is not blocked by the presence of elevated KCl. The unusually short and flattened appearance of neurites in these cultures suggests that the M6 antibody selectively affects neurite extension. Time-lapse cinematography of anti-M6-treated neurons reveals no apparent effect on movement of lamellipodia and filopodia of growth cones. Only the overall extension of the neurite appears to be inhibited. M6 antigen is a 35 kD glycoprotein that can be isolated from a deoxycholate- (DOC) solubilized membrane fraction from adult mouse brain.