REPRODUCIBLE HIGH-YIELD OF RAT ISLETS BY STATIONARY INVITRO DIGESTION FOLLOWING PANCREATIC DUCTAL OR PORTAL VENOUS COLLAGENASE INJECTION
REPRODUCIBLE HIGH-YIELD OF RAT ISLETS BY STATIONARY INVITRO DIGESTION FOLLOWING PANCREATIC DUCTAL OR PORTAL VENOUS COLLAGENASE INJECTION
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DOI：
10.1097/00007890-198705000-00024
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发表时间：
1987-05-01
期刊：
影响因子：
6.2
通讯作者：
MONACO, AP
中科院分区：
文献类型：
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作者：
GOTOH, M;MAKI, T;MONACO, AP
用胶原酶溶液扩张胰腺，然后在体外静止消化，可产生大量完整的胰岛。在大鼠中，我们比较了两种胶原酶注射途径，胰腺导管(PD)和门静脉(PV)，比较了胰岛产量、体外胰岛素分泌能力和体内功能生存能力。胰岛产量PD法(n=11)大于PV法(n=8)(682.±-)。27比417。+-。每个胰腺39个，P<；0.025)。从PD胰岛释放的胰岛素对16.7 mM葡萄糖的反应在培养后逐渐增加，3.2+。0.8 ng/10个胰岛/30分钟(新鲜)至12.3+-。2.1(24小时培养)。相反，在培养的前6小时，PV胰岛的胰岛素释放增加，但在培养24小时后减少。光镜下，PD胰岛结构完好，内分泌细胞健康，而PV胰岛毛细血管网络扩张，内分泌细胞连续性扭曲。将100个PD胰岛移植到链脲佐菌素诱导的糖尿病B6AF1小鼠(n=8)，所有受体小鼠的血糖均恢复正常(
Pancreatic distension with collagenase solution followed by stationary in vitro digestion yields large numbers of intact islets. We compared in rats two routes of collagenase injection, pancreatic ductal (PD) and portal venous (PV), for islet yield, in vitro insulin secretory capacities, and in vivo functional viability. The islet yield the PD method (n=11) was greater than that in the PV method (n=8) (682 .+-. 27 vs. 417 .+-. 39 per pancreas, P < 0.025). The insulin release from the PD islets in response to 16.7 mM glucose increased gradually following culture, 3.2 .+-. 0.8 ng/10 islets/30 min (fresh) to 12.3 .+-. 2.1 (24-hr culture). In contrast, insulin release from the PV islets increased during the first 6 hr of culture, but decreased after 24 hr in culture. Under electronmicroscopic examination, the PD islets revealed a well preserved structure with healthy endocrine cells, while the PV islets showed a dilated capillary network and distorted endocrine cell continuity. When 100 PD islets were transplanted into streptozotocin-induced diabetic B6AF1 mice (n=8), all the recipient mice restored normoglycemia (