RESPIRATION CORRECTIONS FOR BACTERIAL UPTAKE OF DISSOLVED ORGANIC COMPOUNDS IN NATURAL WATERS1
RESPIRATION CORRECTIONS FOR BACTERIAL UPTAKE OF DISSOLVED ORGANIC COMPOUNDS IN NATURAL WATERS1
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天然水中细菌吸收溶解有机化合物的呼吸修正1
DOI：
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发表时间：
1969
期刊：
影响因子：
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通讯作者：
C. C. Crawford
中科院分区：
文献类型：
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作者：
J. Hobbie;C. C. Crawford
利用~（14）C标记的有机化合物对水体中异养微生物的摄取研究已被广泛应用于沃茨中异养微生物的研究。然而，如果要测量有机化合物的通量率，在培养过程中14 CO2的损失成为一个重要的误差来源。提出了一种在密闭系统中进行实验，经压气酸化后收集14CO_2的方法。色谱纸上的苯乙胺为吸收剂，通过液体闪烁法对色谱纸进行计数。对池塘水中19种化合物的研究表明，60%（天冬氨酸）至8%（精氨酸）的标记物质进入微生物后被呼吸。
The uptake of 14C-labeled organic compounds has been used by many workers to study heterotrophic microorganisms in natural waters. However, if flux rates of organic compounds are to be measured, the loss of 14CO2 during incubation becomes an important source of error. A method is proposed in which the experiment is run in a closed system and the 14CO2 collected after killing and acidification. Phenethylamine on chromatographic paper is the absorbing agent and the paper is counted by liquid scintillation. Studies of 19 compounds from pond water showed that 60% (aspartic acid) to 8% (arginine) of the labeled material entering the microorganisms was respired.