Crystallization of hen egg-white avidin in a tetragonal form.
Crystallization of hen egg-white avidin in a tetragonal form.
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鸡蛋清亲和素的四方结晶。
DOI：
10.1016/0022-2836(84)90253-5
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发表时间：
1984
影响因子：
5.6
通讯作者：
M. Malcovati
中科院分区：
文献类型：
--
作者：
G. Gatti;M. Bolognesi;A. Coda;F. Chiolerio;E. Filippini;M. Malcovati
用硫酸铵溶液在pH 5.7下结晶出蛋清亲和素。晶体属于四边形空间群P4 2 2 1 2，胞边a= b= 79.6 a￣，c= 84.3 a￣。假设每个亲和素单体的分子量为15,600，这种晶体形式与不对称单元中二聚体的存在兼容，并且适合于高分辨率的晶体学结构研究。
Hen egg-white avidin has been crystallized at pH 5.7 from ammonium sulfate solutions. The crystals belong to the tetragonal space group P4 2 2 1 2, with unit cell edges a= b= 79.6 A ̊, c= 84.3 A ̊. Assuming a molecular weight of 15,600 per avidin monomer, this crystal form is compatible with the presence of a dimer in the asymmetric unit, and is suitable for a crystallographic structural investigation at high resolution.