Interleukin-15 interactions with interleukin-15 receptor complexes: Characterization and species specificity
Interleukin-15 interactions with interleukin-15 receptor complexes: Characterization and species specificity
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DOI：
10.1006/cyto.2002.1989
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发表时间：
2002-11-07
期刊：
影响因子：
3.8
通讯作者：
Park, LS
中科院分区：
文献类型：
--
作者：
Eisenman, J;Ahdieh, M;Park, LS
白细胞介素 (IL-) 2 和 IL-15 共享 IL-2 受体 betagamma(c) 亚基 (IL-2Rbetagamma(c))，但具有特异性、独特的 a 受体亚基。我们研究了人类 (hu)、猿猴 (si) 和小鼠 (mu) IL-15 的物种特异性，发现 hu 和 A IL-15 在所有研究的系统中表现相似。在存在或不存在IL-2Rbetagamma(c)的情况下，Hu和mu IL-15以相同的高亲和力结合hu或mu IL-15Rα，并且对包含所有三个亚基的细胞表现出相似的增殖活性。然而，在不存在IL-15Rα的情况下使用IL-2Rbetagamma(c)的体外和体内系统中，hu和mu IL-15的比活性存在定量差异。这些数据表明 hu IL-15 可用于小鼠模型系统，但在比较不同物种细胞因子的功效和毒性时必须小心。 (C) 2002 Elsevier science Ltd. 保留所有权利。
Interleukin (IL-) 2 and IL-15 share the IL-2 receptor betagamma(c) subunits (IL-2Rbetagamma(c)) but have specific, unique a receptor subunits. We studied species specificity of human (hu), simian (si), and mouse (mu) IL-15 and found that hu and A IL-15 behaved similarly in all systems investigated. Hu and mu IL-15 bound hu or mu IL-15Ralpha with equal high affinity in the presence or absence of IL-2Rbetagamma(c), and exhibited similar proliferative activities on cells containing all three subunits. However, quantitative differences were noted in the specific activity of hu and mu IL-15 in both in vitro and in vivo systems utilizing IL-2Rbetagamma(c) in the absence of IL-15Ralpha. These data show that hu IL-15 may be used in mouse model systems, however care must be taken when comparing the efficacy and toxicity of cytokines across species. (C) 2002 Elsevier science Ltd. All rights reserved.