Effects of estrogen on growth hormone pulsatility in peripheral blood and neuropeptide profiles in the cerebrospinal fluid of goats.
Effects of estrogen on growth hormone pulsatility in peripheral blood and neuropeptide profiles in the cerebrospinal fluid of goats.
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雌激素对山羊外周血中生长激素脉动和脑脊液中神经肽谱的影响。
DOI：
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发表时间：
2011
影响因子：
1.8
通讯作者：
M. Nishihara
中科院分区：
文献类型：
--
作者：
T. Yonezawa;K. Mogi;J. Li;Ryuji Sako;N. Manabe;K. Yamanouchi;M. Nishihara
我们以前曾报道，生长激素（GH）脉冲与神经肽Y（NPY）的配置文件在第三脑室的芝山羊脑脊液（CSF）呈负相关。此外，虽然大多数GH脉冲与GH释放激素（GHRH）脉冲一致，但GH和生长抑素（SRIF）水平之间没有相关性。本研究旨在阐明雌激素（1.0 mg/头）皮下注射给卵巢切除山羊时，GH脉冲和CSF中这些神经肽水平之间的关系。从注射前6 h至注射后12 h，每15 min采集一次CSF和血浆样本，共18 h。采用放射免疫法测定外周血GH水平和脑脊液GHRH、SRIF、NPY水平。通过ULTRA算法和互相关分析评估脉搏/谷值特征和相关性。注射雌二醇前，GHRH脉冲与GH脉冲呈明显的一致性，NPY谷值与GH脉冲呈负一致性。注射雌二醇后6 ~ 12 h，GH脉冲的幅度和曲线下面积（AUC）均显著增加。CSF中GHRH脉冲的持续时间和AUC也增加，并且观察到GHRH与GH更强的同步性。与此相反，NPY的基线显著降低，GH脉冲与NPY谷值之间的负相关消失。SRIF谷值参数变化不明显。这些观察结果表明，雌激素通过增强GHRH脉冲和降低NPY水平来增强山羊GH的分泌模式。
We previously reported that growth hormone (GH) pulses were negatively associated with neuropeptide Y (NPY) profiles in cerebrospinal fluid (CSF) of the third ventricle of Shiba goats. In addition, while most GH pulses were coincident with GH-releasing hormone (GHRH) pulses, there was no correlation between GH and somatostatin (SRIF) levels. The present study was performed to elucidate the relationship between GH pulses and these neuropeptide levels in CSF when estradiol (1.0 mg/head) was subcutaneously administered to ovariectomized goats. CSF and plasma samples were collected every 15 min for 18 h (from 6 h before to 12 h after injection). GH levels in peripheral blood and GHRH, SRIF and NPY levels in CSF were measured by radioimmunoassay. Pulse/trough characteristics and correlations were assessed by the ULTRA algorithm and cross-correlation analysis. Before estradiol was injected, significant coincidence was found between GHRH pulses and GH pulses, and negative coincidence was found between NPY troughs and GH pulses. Six to 12 h after estradiol injection, the amplitude and area under the curve (AUC) of the GH pulses were markedly increased. The duration and AUC of the GHRH pulses in the CSF were also increased, and stronger synchrony of GHRH with GH was observed. In contrast, the baseline of NPY was significantly decreased, and the negative correlation between the GH pulses and NPY troughs disappeared. The parameters of SRIF troughs were not clearly changed. These observations suggest that estrogen enhances the pattern of secretion of GH in the goat via enhancement of GHRH pulses and decrease of NPY levels.
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在推定的生长抑素撤退环境中，补充雌二醇可增强重组人 GH 释放激素 1,44-酰胺 分泌生长激素 (GH) 的次最大前馈驱动。
DOI：
10.1210/jc.2003-030410
发表时间：
2003
期刊：
The Journal of clinical endocrinology and metabolism
影响因子：
--
作者：
Veldhuis,JohannesD;Evans,WilliamS;Bowers,CyrilY
通讯作者：
Bowers,CyrilY
神经肽 Y 通过刺激生长抑素释放来抑制生长激素释放，具有显着的生理作用。
影响因子：
4.8
作者：
Rettori,V;Milenkovic,L;Aguila,MC;McCann,SM
通讯作者：
McCann,SM
去势恒河猴第三脑室脑脊液中促性腺激素释放激素脉冲：与黄体生成素脉冲的相关性。
影响因子：
4.8
作者：
VanVugt,DA;Diefenbach,WD;Alston,E;Ferin,M
通讯作者：
Ferin,M
有证据表明，释放到垂体-门静脉循环中的神经肽 Y (NPY) 参与促性腺激素释放激素 (GnRH) 的作用。
影响因子：
4.8
作者：
Sutton,SW;Toyama,TT;Otto,S;Plotsky,PM
通讯作者：
Plotsky,PM
在卵巢类固醇诱导的黄体生成素激增之前，下丘脑神经肽-Y 基因表达增加。
影响因子：
4.8
作者：
Sahu,A;Crowley,WR;Kalra,SP
通讯作者：
Kalra,SP