Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels
Label-free colorimetric detection of trace cholesterol based on molecularly imprinted photonic hydrogels
复制标题
基于分子印迹光子水凝胶的痕量胆固醇无标记比色检测
DOI：
10.1039/c1jm14230e
复制
发表时间：
2011-01-01
影响因子：
--
通讯作者：
Peng, Hailong
中科院分区：
文献类型：
--
作者：
Li, Jinhua;Zhang, Zhong;Peng, Hailong
将分子印迹技术与光子晶体相结合，构建了一种新型的胆固醇比色传感器。以胆固醇为模板分子，采用非共价自组装方法制备了分子印迹光子水凝胶（MIPH）膜，并通过扫描电子显微镜（SEM）表征了优化的印迹条件下MIPH膜的三维有序大孔结构。讨论了影响胆固醇再结合的各种因素，沿着通过紫外-可见光谱和电化学阻抗谱的估计，对其类似物豆甾醇和麦角甾醇的识别特异性进行了研究。MIPH膜在结合胆固醇后不到2分钟内产生直接自报告的显著可读的光学信号。胆固醇浓度的比色测量强烈依赖于MIPH的布拉格衍射峰的蓝移效应随着胆固醇量的增加而逐渐扩大的事实。检测水平接近10 - 13 g mL-1，这与荧光测量相当。该传感器同时具有高选择性、高灵敏度、高稳定性、操作简便、无需标记等优点，有望用于痕量胆固醇的现场快速检测。
A novel colorimetric sensor for cholesterol assay was constructed by combining a molecular imprinting technique with photonic crystals. The molecularly imprinted photonic hydrogel (MIPH) film was prepared by a non-covalent, self-assembly approach using cholesterol as a template molecule, and exhibited a highly ordered three-dimensional macroporous structure characterized by scanning electron microscopy under the optimized imprinting conditions. Various factors affecting rebinding of cholesterol are discussed along with recognition specificity studies on its analogues of stigmasterol and ergosterol through estimation of UV-Vis and electrochemical impedance spectroscopy. The MIPH film generated a significantly readable optical signal directly self-reporting within less than 2 min upon binding cholesterol. The colorimetric measurement of cholesterol concentration strongly relies on the fact that the blue shift effect of the Bragg diffraction peak of the MIPH is gradually enlarged with the increase of cholesterol amounts. The detection level approached 10−13 g mL−1, which is comparable to that of fluorescence measurements. The simultaneous possession of high selectivity, high sensitivity, high stability, easy operation and being label-free enables this sensor to be potentially applicable for rapid on-site detection of trace cholesterol.