Altitudinal and seasonal changes of phenolic compounds in Buxus sempervirens leaves and cuticles
Altitudinal and seasonal changes of phenolic compounds in Buxus sempervirens leaves and cuticles
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DOI：
10.1016/j.plaphy.2013.06.012
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发表时间：
2013-09-01
影响因子：
6.5
通讯作者：
Verdaguer, D.
中科院分区：
文献类型：
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作者：
Bernal, M.;Llorens, L.;Verdaguer, D.
植物叶片中酚类化合物含量的变化与植物生长环境的UV-B变化有关。在此背景下，我们的目的是研究：a）是否季节性和海拔高度的变化，在黄杨的酚类化合物的含量。叶片和冠层可能与UV-B水平的自然波动有关，以及B）可能使用特定的酚类化合物作为环境UV-B水平的生物标志物。为了实现这些目标，我们抽样，每三个月在一年中，叶B。沿着海拔梯度生长的常绿植物。在最低和最高海拔，我们还进行了紫外线排斥实验，以辨别所观察到的变化是否可以归因于UV-B的自然变化。结果表明，总酚含量在6月低于其他采样日期，这表明叶个体发育，而不是UV-B对这些化合物的叶含量的影响。在海拔梯度上，全叶总酚酸和新木脂素含量随海拔升高而增加，叶表皮总黄酮含量随海拔升高而减少。然而，我们的UV排斥处理对这些化合物的含量没有显著影响，这表明所观察到的沿沿着海拔梯度的变化将响应于其他因素，而不是UV-B。同时，在B的叶片和表皮中未发现酚类化合物。sempervirens，可以被认为是环境UV-B水平的生物标志物。(c)2013年Elsevier Masson SAS。All rights reserved.
The variation in the leaf content of phenolic compounds has been related to the UV-B changes of the environment in which plants grow. In this context, we aimed to investigate: a) whether the seasonal and altitudinal changes in the content of phenolic compounds of Buxus sempervirens L. leaves and cuticles could be related to the natural fluctuations in UV-B levels and b) the possible use of specific phenolic compounds as biomarkers of ambient UV-B levels. To achieve these goals we sampled, every three months during one year, leaves of B. sempervirens along an altitudinal gradient. At the lowest and the highest altitudes, we also conducted a UV-exclusion experiment to discern whether the observed changes could be attributed to the natural variation in UV-B. Results show that total phenolic content of leaves was lower in June than in the other sampling dates, which suggests a leaf ontogenic rather than a UV-B effect on the leaf content of these compounds. Regarding the elevational gradient, the overall amount of phenolic acids and neolignan of entire leaves increased with altitude while the total amount of flavonoids in leaf cuticles decreased. However, the lack of a significant effect of our UV-exclusion treatment on the content of these compounds suggests that the observed variations along the altitudinal gradient would respond to other factors rather than to UV-B. Concomitantly, we did not find any phenolic compound in leaves or cuticles of B. sempervirens that could be considered as a biomarker of ambient UV-B levels. (c) 2013 Elsevier Masson SAS. All rights reserved.