Effects of advanced glycation end products on dental pulp calcification
Effects of advanced glycation end products on dental pulp calcification
复制标题
晚期糖基化终末产物对牙髓钙化的影响
DOI：
10.1111/odi.13792
复制
发表时间：
2021
期刊：
影响因子：
3.8
通讯作者：
Araki Tsutomu
中科院分区：
文献类型：
--
作者：
Sugiyama Keita;Miura Jiro;Shimizu Masato;Takashima Aoi;Matsuda Yusuke;Kayashima Hiroki;Okamoto Motoki;Nagashima Tadashi;Araki Tsutomu
本研究的主要目的是阐明晚期糖基化终产物（AGEs）对体外培养的大鼠牙髓细胞（RDPCs）钙化的影响，并探讨糖基化胶原的结晶能力。为了研究AGEs对RDPC的影响，我们进行了WST-1和乳酸脱氢酶测定;碱性磷酸酶，茜素红S和免疫组化染色;以及真实的实时定量逆转录PCR。此外，我们对糖化胶原蛋白进行了结晶实验。所有的微观结构进行了分析，使用扫描电子显微镜/能量色散X射线光谱和透射电子显微镜/衍射图案analysis.ResultsAGEs不影响RDPCs的增殖或分化，但提高钙化率和细胞毒性。在这些钙化中没有涉及主要的钙化相关基因或蛋白质，并且发现糖化胶原蛋白表现出负极性并形成磷酸钙晶体。由于细胞周围离子浓度的剧烈变化导致的细胞毒性导致营养不良性钙化，假设代表糖尿病牙髓calcifiers.ConclusionGlycated胶原蛋白-含有AGEs的一个方面提供了一个培育环境结晶和RDPC的早期钙化有显着的影响。
ObjectiveThe main aim of this study was to elucidate the effects of advanced glycation end products (AGEs) on the calcification of cultured rat dental pulp cells (RDPCs) and to investigate the crystallisation ability of glycated collagen.Materials and MethodsAGEs were prepared via non‐enzymatic glycation of a dish coated with type I collagen usingdl‐glyceraldehyde. To investigate the effects of AGEs on RDPCs, we performed WST‐1 and lactate dehydrogenase assays; alkaline phosphatase, Alizarin Red S and immunohistochemical staining; and real‐time quantitative reverse transcription PCR. In addition, we performed crystallisation experiments on glycated collagen. All microstructures were analysed using scanning electron microscopy/energy‐dispersive X‐ray spectroscopy and transmission electron microscopy/diffraction pattern analysis.ResultsAGEs did not affect the proliferation or differentiation of RDPCs, but enhanced the calcification rate and cytotoxicity. No major calcification‐related genes or proteins were involved in these calcifications, and glycated collagen was found to exhibit a negative polarity and form calcium phosphate crystals. Cytotoxicity due to drastic changes in the concentration of pericellular ions led to dystrophic calcification, assumed to represent an aspect of diabetic pulp calcifications.ConclusionGlycated collagen‐containing AGEs provide a nurturing environment for crystallisation and have a significant effect on the early calcification of RDPCs.