Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars
Two New Members of CsFEXs Couple Proton Gradients to Export Fluoride and Participate in Reducing Fluoride Accumulation in Low-Fluoride Tea Cultivars
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CsFEX 的两名新成员耦合质子梯度以输出氟化物并参与减少低氟茶品种中的氟化物积累。
DOI：
10.1021/acs.jafc.0c03444
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Legong Li
中科院分区：
文献类型：
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作者：
Jiali Song;Congcong Hou;Jiangxin Guo;Qi Niu;Xiaohan Wang;Zhijie Ren;Qian Zhang;Changxin Feng;Liangyu Liu;Wang Tian;Legong Li
不同品种茶叶中氟化物的积累存在显着差异。然而，分子基础和机制仍然很大程度上未知。在这里，我们报道了CsFEX（茶花氟输出基因）的两个基因CsFEX1和CsFEX2，将氟转运出细胞，减轻细胞氟毒素，并拯救酵母突变体（FEX1ΔFEX2Δ）和拟南芥突变体（fex），因为它们的外排活性与质子梯度耦合。进一步分析发现，CsFEX1 和 CsFEX2 在酵母和拟南芥细胞中均定位于质膜。与CsFEX1相比，即使在低pH值下，CsFEX2也能更有效地降低酵母和拟南芥中的氟毒性。氟处理诱导的CsFEX2在低氟品种（云康10）中比在高氟品种（平阳特早茶）中高约10倍，这表明CsFEX2可能在减少茶叶中氟化物积累方面发挥着关键作用。
The accumulation of fluoride in tea leaves from various cultivars exhibits significant differences. However, the molecular basis and mechanism remain largely unknown. Here, we reported that two genes ofCsFEX(fluoride export genes inCamellia sinensis),CsFEX1andCsFEX2, transport fluoride out of cells, alleviate the cellular fluoride toxin, and rescue the yeast mutant (FEX1ΔFEX2Δ) andArabidopsismutant (fex), as their efflux activities are coupled with proton gradients. Further analysis found that CsFEX1 and CsFEX2 localize to the plasma membrane both in yeast andArabidopsiscells. CsFEX2 is more effective to reduce fluoride toxicity in yeast andArabidopsiscompared with CsFEX1 even at low pH.CsFEX2induced by fluoride treatment is around tenfold higher in a low-fluoride cultivar (Yunkang 10) than that in a high-fluoride cultivar (Pingyang Tezaocha), suggesting thatCsFEX2possibly plays a critical role in reducing fluoride accumulation in tea leaves.