Identification of stem cells after transplantation.
Identification of stem cells after transplantation.
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DOI：
10.1007/978-1-62703-511-8_8
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
Tang, Yaoliang
中科院分区：
文献类型：
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作者：
Wang, Yingjie;Zhang, Lan;Pan, Yaohua;Chen, Lijuan;Weintraub, Neal;Tang, Yaoliang
移植到受伤心脏后识别供体干细胞的能力至关重要。这对于评估干细胞存活和向成熟心血管细胞的谱系分化特别重要。已采用多种方法来追踪供体干细胞，包括荧光染料和荧光蛋白基因转移。在这里，我们将诱导使用慢病毒介导的绿色荧光蛋白（GFP）来监测移植后供体干细胞的命运的方案。
The ability to identify the donor stem cells following transplantation into injured hearts is critical. This is particularly important in evaluating stem cell survival and lineage differentiation into mature cardiovascular cells. Several approaches have been employed for tracking the donor stem cells, including fluorescent dyes and fluorescent protein gene transfer. Here, we will induce a protocol using lentivirus-mediated green fluorescent protein (GFP) to monitor the fate of donor stem cells following transplantation.