The axon guidance gene lola is required for programmed cell death in the Drosophila ovary
The axon guidance gene lola is required for programmed cell death in the Drosophila ovary
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DOI：
10.1016/j.ydbio.2007.01.029
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发表时间：
2007-04-15
影响因子：
2.7
通讯作者：
McCall, Kimberly
中科院分区：
文献类型：
--
作者：
Bass, B. Paige;Cullen, Kristen;McCall, Kimberly
在果蝇中发现了一个基因，编码参与轴突导向的几种选择性剪接转录因子。在这里，我们报告说，Iola在卵巢细胞程序性死亡中也起着至关重要的作用。Iola突变体生殖系克隆显示出高百分比的卵室，其具有持续超过第13阶段的滋养细胞核，表明发育滋养细胞死亡的阻断。突变体还显示出对卵子发生中期因饥饿而发生的诱导程序性细胞死亡的破坏。进一步的表征表明，Iola生殖系克隆表现出异常的核组织，随着年龄的增长变得越来越严重。染色质呈弥散状，不能正确凝聚或在垂死的滋养细胞中发生DNA断裂。大量的核物质积累在含有Iola生殖系克隆的老年果蝇的卵巢中。我们建议，Iola是必要的完全染色质凝聚过程中发生的程序性细胞死亡在卵巢。等位基因不同的强度，他们的表型，但有趣的是，他们的卵巢表型的严重程度是独立的强度，他们的神经元表型，这表明个别Iola亚型在卵巢和神经系统的差异要求。(c)2007爱思唯尔公司All rights reserved.
longitudinals-lacking (Iola) was identified in Drosophila as a gene encoding several alternatively spliced transcription factors involved in axon guidance. Here we report that Iola also plays a critical role in programmed cell death in the ovary. Iola mutant germline clones show a high percentage of egg chambers with nurse cell nuclei persisting past stage 13, indicating a block in developmental nurse cell death. Mutants also show a disruption in the induced programmed cell death that occurs during mid-oogenesis in response to starvation. Further characterization revealed that Iola germline clones exhibit abnormal nuclear organization which becomes increasingly severe with age. Chromatin appears diffuse and fails to condense properly or undergo DNA fragmentation in dying nurse cells. Masses of nuclear material accumulate in the ovaries of older flies containing Iola germline clones. We propose that Iola is necessary for complete chromatin condensation which occurs during programmed cell death in the ovary. Alleles differed in the strength of their phenotypes but interestingly, the severity of their ovarian phenotypes was independent of the strength of their neuronal phenotypes, suggesting a differential requirement for individual Iola isoforms in the ovary and nervous system. (c) 2007 Elsevier Inc. All rights reserved.