Hepatic (Na+,K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies.
Hepatic (Na+,K+)-ATPase: a current view of its structure, function and localization in rat liver as revealed by studies with monoclonal antibodies.
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肝 (Na ,K )-ATP 酶：单克隆抗体研究揭示了其在大鼠肝脏中的结构、功能和定位的最新观点。
DOI：
10.1002/hep.1840050327
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Keller,GA
中科院分区：
文献类型：
--
作者：
Leffert,HL;Schenk,DB;Hubert,JJ;Skelly,H;Schumacher,M;Ariyasu,R;Ellisman,M;Koch,KS;Keller,GA
最近使用单克隆抗体（1-3）进行的免疫细胞化学研究再次引发争议（Yousef，I.例如，胃肠病学1984; 86：1632-1633，通信和Blitzer B。和Boyer，J.，Gastroenterology 1984; 87：1206-1207，Correspondence），涉及大鼠肝细胞（Na+，K+）-ATP酶的拓扑结构（4-6）。除了这种酶（所谓的“钠泵”）对于调节细胞体积（7）、分化和增殖（8）至关重要的事实之外，其在质膜中的位置的问题与胆汁酸和水从窦腔运输到小管腔的机制特别相关。这些过程的理论取决于钠泵在细胞表面的分布位置和程度，细胞表面与这些空间中的一个或两个相邻（4-6，9，10，Yousef I.例如，同上，Correspondence and Blitzer，B.和Boyer。J.，同上，在这份报告中，我们总结了实验结果，这些结果强烈表明肝细胞钠泵的分布是不对称的，与窦状隙和侧部区域相反，小管上的泵部位密度增加（2，3，11，Leffert，H.例如，未发表的评论）。本文将对肝脏（Na+，K+）-ATP酶的丰度和结构特性进行新的观察。一些定量和定性的影响，这些意见进行了讨论。
Recent immunocytochemical studies with monoclonal antibodies (1-3) have revived controversy (Yousef, I. et al., Gastroenterology 1984; 86: 1632-1633, Correspondence and Blitzer B. and Boyer, J., Gastroenterology 1984; 87: 1206-1207, Correspondence) concerning the topology of rat hepatocyte (Na+, K+)-ATPase (4-6). Apart from the fact that this enzyme (the so-called “sodium pump”) is crucial for regulating cellular volume (7), differentiation and proliferation (8), the issue of its whereabouts in the plasma membrane is particularly relevant to the mechanism of bile acid and water transport from sinusoidal to canalicular spaces. Theories of these processes depend on where and to what extent the sodium pump is distributed in cell surfaces adjacent to either one or both of these spaces (4-6, 9, 10, Yousef I. et al., op cit., Correspondence and Blitzer, B. and Boyer. J., op cit., Correspondence).In this report, we summarize experimental results which strongly suggest that hepatocyte sodium pumps are distributed asymmetrically, with an increased pump site density on canalicular as opposed to sinusoidal and lateral domains (2, 3, 11, Leffert, H. et al., unpublished observations). New observations on the abundance and structural properties of hepatic (Na+, K+)-ATPase will be presented. Some of the quantitative and qualitative implications of these observations are discussed.