Cloning and sequence analysis of complementary DNA encoding an aberrantly rearranged human T-cell gamma chain.
Cloning and sequence analysis of complementary DNA encoding an aberrantly rearranged human T-cell gamma chain.
复制标题
编码异常重排的人类 T 细胞伽马链的互补 DNA 的克隆和序列分析。
DOI：
10.1073/pnas.83.8.2619
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发表时间：
1986
影响因子：
11.1
通讯作者：
Strominger,JL
中科院分区：
文献类型：
--
作者：
Dialynas,DP;Murre,C;Quertermous,T;Boss,JM;Leiden,JM;Seidman,JG;Strominger,JL
编码人T细胞γ链的互补DNA（cDNA）已被克隆和测序。在可变区和连接区的连接处，相对于鼠γ链cDNA序列，人cDNA序列中存在两个核苷酸的明显缺失，同时导致框内终止密码子的产生和翻译移码。由于这个原因，这里呈现的序列编码异常重排的人类T细胞γ链。在推导的人和鼠γ链氨基酸序列之间存在几个令人惊讶的差异。这些包括可变区同源性差，恒定区的离散片段同源性差，该恒定区与外显子CII的预期连接点精确结合，以及人序列中存在5个潜在的N-连接糖基化位点。
Complementary DNA (cDNA) encoding a human T-cell gamma chain has been cloned and sequenced. At the junction of the variable and joining regions, there is an apparent deletion of two nucleotides in the human cDNA sequence relative to the murine gamma-chain cDNA sequence, resulting simultaneously in the generation of an in-frame stop codon and in a translational frameshift. For this reason, the sequence presented here encodes an aberrantly rearranged human T-cell gamma chain. There are several surprising differences between the deduced human and murine gamma-chain amino acid sequences. These include poor homology in the variable region, poor homology in a discrete segment of the constant region precisely bounded by the expected junctions of exon CII, and the presence in the human sequence of five potential sites for N-linked glycosylation.