3D cell aggregate printing technology and its applications.
3D cell aggregate printing technology and its applications.
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3D细胞聚合体打印技术及其应用
DOI：
10.1042/ebc20200128
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发表时间：
2021-07
影响因子：
6.4
通讯作者：
Seunggyu Jeon;Se-Hwan Lee;Saeed B Ahmed;Jonghyeuk Han;S. Heo;Hyunwook Kang
中科院分区：
文献类型：
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作者：
Seunggyu Jeon;Se-Hwan Lee;Saeed B Ahmed;Jonghyeuk Han;S. Heo;Hyunwook Kang
各种细胞聚集体培养技术已被开发并积极应用于组织工程和器官芯片。然而，传统的养殖技术是劳动密集型的，其结果高度依赖于用户。此外，这些技术不能用于生产三维（3D）复杂组织。在这方面，3D细胞聚集体打印技术由于其3D可加工性而引起了许多研究人员的越来越多的关注。该技术允许以自动化方式使用多种类型的细胞聚集体制造3D自由形态构建体。技术进步导致了在3D空间中具有约20 μm的高分辨率的打印技术的发展。还引入了可以在毫秒内打印细胞聚集体的高速打印技术。已开发的聚合物打印技术正被积极应用于生产各种类型的工程组织。尽管已经开发了各种类型的高性能打印技术，但在制造模拟天然组织的结构和功能的工程组织方面仍然存在一些技术障碍。本文综述了3D细胞聚集体打印技术的核心重要性和当前的技术水平，以及它们在组织/疾病模型、人工组织和药物筛选平台上的应用。本文还讨论了剩余的障碍和未来的发展方向的印刷工艺。
Various cell aggregate culture technologies have been developed and actively applied to tissue engineering and organ-on-a-chip. However, the conventional culture technologies are labor-intensive, and their outcomes are highly user dependent. In addition, the technologies cannot be used to produce three-dimensional (3D) complex tissues. In this regard, 3D cell aggregate printing technology has attracted increased attention from many researchers owing to its 3D processability. The technology allows the fabrication of 3D freeform constructs using multiple types of cell aggregates in an automated manner. Technological advancement has resulted in the development of a printing technology with a high resolution of approximately 20 μm in 3D space. A high-speed printing technology that can print a cell aggregate in milliseconds has also been introduced. The developed aggregate printing technologies are being actively applied to produce various types of engineered tissues. Although various types of high-performance printing technologies have been developed, there are still some technical obstacles in the fabrication of engineered tissues that mimic the structure and function of native tissues. This review highlights the central importance and current technical level of 3D cell aggregate printing technology, and their applications to tissue/disease models, artificial tissues, and drug-screening platforms. The paper also discusses the remaining hurdles and future directions of the printing processes.