LncRNA HOXA11-AS promotes cell growth by sponging miR-24-3p to regulate JPT1 in prostate cancer
LncRNA HOXA11-AS promotes cell growth by sponging miR-24-3p to regulate JPT1 in prostate cancer
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DOI：
10.26355/eurrev_202107_26377
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发表时间：
2021-01-01
影响因子：
3.3
通讯作者：
Liao, G-L
中科院分区：
文献类型：
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作者：
Cheng, Y.;Xiong, H-Y;Liao, G-L
目的：长链非编码RNA（lncRNA）在肿瘤的发生、发展过程中起着重要的调控作用. HOXA 11反义RNA（HOXA 11-AS）是一种致癌的lncRNA，但其在前列腺癌（PCa）中的作用尚不清楚。通过MTT法、克隆形成实验、transwell侵袭实验和流式细胞术分析HOXA 11-AS在PCa中的生物学作用。采用补救实验研究HOXA 11-AS在PCa中的作用机制。HOXA 11-AS基因沉默可显著抑制PCa细胞的增殖、集落形成、侵袭和促进细胞凋亡。相反，强制HOXA 11-AS表达对癌细胞行为产生相反的影响。此外，我们发现HOXA 11-AS 1作为竞争性内源性RNA（ceRNA）通过海绵状microRNA-24- 3 p（miR-24- 3 p）调节Jupiter微管相关同源物1（JPT 1）。结论：新发现的HOXA 11-AS/miR-24 - 3 p/JPT 1轴可能为更好地调控PCa提供新的角度。
OBJECTIVE: Long noncoding RNA (lncRNA) was found to play crucial roles in regulating cancer progression. HOXA11 anti-sense RNA (HOXA11-AS) was reported to serve an oncogenic lncRNA in cancers but its role in prostate cancer (PCa) remains to be explored.MATERIALS AND METHODS: Expression levels of HOXA11-AS in PCa tissues and cells were analyzed with quantitative Real-Time PCR method. MTT assay, colony formation assay, transwell invasion assay, and flow cytometry assay were conducted to explore the biological roles of HOXA11-AS in PCa. Rescue experiments were conducted to investigate mechanisms of HOXA11-AS in regulating PCa progression.RESULTS: We revealed that HOXA11-AS was up-regulated in PCa. Silencing of HOXA11-AS significantly inhibited PCa cell proliferation, colony formation, invasion, and promoted apoptosis in vitro. On the contrary, forcing of HOXA11-AS expression caused opposite effects on cancer cell behaviors. Furthermore, we showed that HOXA11-AS1 serves as a competing endogenous RNA (ceRNA) to regulate Jupiter microtubule associated homolog 1 (JPT1) via sponging microRNA-24-3p (miR-24-3p). Functionally, the overexpression of miR-24-3p or knockdown of JPT1 could partially reverse the effects of HOXA11-AS overexpression on PCa cell behaviors.CONCLUSIONS: This newly identified HOXA11-AS/miR-24-3p/JPT1 axis may provide novel angle for the better control of PCa.