Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection
Rapid analysis of triterpenic acids by liquid chromatography using porous graphitic carbon and evaporative light scattering detection
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DOI：
10.1002/jssc.200401764
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发表时间：
2004-08-01
影响因子：
3.1
通讯作者：
Lafrosse, M
中科院分区：
文献类型：
--
作者：
Bérangère, C;Caussarieu, N;Lafrosse, M
提出了一种以多孔石墨碳为载体、有机溶剂混合物为流动相的液相色谱分析三萜酸的原创体系。桦木酸、熊果酸、齐墩果酸、18α-和18β-甘草次酸的分离在短时间内完成，并通过蒸发光散射检测作为通用检测方法进行监测。分子建模研究表明，对选择性的主要贡献来自于以产物偶极矩为特征的静电相互作用。
An original system which uses Porous Graphitic Carbon as support and a mixture of organic solvents as mobile phase is proposed for the analysis of triterpenic acids by liquid chromatography. The separation of betulinic acid, ursolic acid, oleanolic acid, and 18alpha- and 18beta-glycyrrhetinic acids was carried out within a short time and monitored by evaporative light scattering detection as universal detection method. Molecular modelling studies show that the main contribution to the selectivity comes from the electrostatic interaction characterised by the dipole moment of the products.