Functional characterization of DiMMS21, a SUMO ligase from Desmodium intortum
Functional characterization of DiMMS21, a SUMO ligase from Desmodium intortum
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DiMMS21（一种来自金钱草的 SUMO 连接酶）的功能表征
DOI：
10.1016/j.plaphy.2019.06.003
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发表时间：
2019
影响因子：
6.5
通讯作者：
Lai Jianbin
中科院分区：
文献类型：
--
作者：
Zhou Xuan;Du Jinju;Liu Yiyang;Yang Chengwei;Lai Jianbin
苏莫化是一种重要的蛋白质修饰，在多种细胞过程中调节底物蛋白质的性质。相扑通过一系列酶催化，通常由相扑E3连接酶刺激。然而，饲料作物中SUMO化的分子功能和调控机制尚不清楚。在这里，我们从牧草豆科牧草大豆中分离到DiMMS21，并对其进行了功能鉴定。DiMMS21在不同的D中普遍表达。侵袭性器官及其编码蛋白存在于细胞质和细胞核中。生物信息学分析表明，DiMMS21含有一个保守的SP环区，这是其活性所必需的。生化证据支持这种蛋白质是一种功能性相扑连接酶的观点。DiMMS21在拟南芥Mms21突变体中表达后，完全修复了根、叶和角果的发育缺陷。子叶变绿和标记基因表达的结果表明，DiMMS21只能部分补充AtMMS21在脱落酸(ABA)反应中的作用。综上所述，我们鉴定了DiMMS21的分子特征，揭示了该相扑连接酶在发育和ABA反应中的潜在作用，加深了我们对饲料作物SUMO化功能的理解。
SUMOylation is an important protein modification that regulates the properties of substrate proteins in a variety of cellular processes. SUMOylation is catalyzed via a cascade of enzymes and is usually stimulated by SUMO E3 ligases. However, the molecular functions and regulatory mechanisms of SUMOylation in forage crops are unknown. Here, we isolated and functionally characterized DiMMS21, a homolog of theArabidopsis thalianaSUMO ligase AtMMS21, from the forage legumeDesmodium intortum.DiMMS21is expressed ubiquitously in variousD. intortumorgans and its encoded protein is found in the cytoplasm and nucleus. Bioinformatics analysis indicated that DiMMS21 contains a conserved SP-RING domain that is required for its activity. Biochemical evidence supports the notion that this protein is a functional SUMO ligase. When expressed in anArabidopsis mms21mutant,DiMMS21completely rescued the defects in root, leaf, and silique development. The results from cotyledon greening and marker gene expression suggested thatDiMMS21can only partially complements the role of AtMMS21 in abscisic acid (ABA) responses. In summary, we characterized the molecular features of DiMMS21 and uncovered potential roles of this SUMO ligase in development and ABA responses, increasing our understanding on the function of SUMOylation in forage crops.