Core Histone Charge and Linker Histone H1 Effects on the Chromatin Structure of Schizosaccharomyces pombe
Core Histone Charge and Linker Histone H1 Effects on the Chromatin Structure of Schizosaccharomyces pombe
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核心组蛋白电荷和连接组蛋白 H1 对粟酒裂殖酵母染色质结构的影响
DOI：
10.1271/bbb.120548
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
K. Takeyasu
中科院分区：
文献类型：
--
作者：
E. Prieto;Kohji Hizume;Kohji Hizume;T. Kobori;T. Kobori;S. Yoshimura;K. Takeyasu
组蛋白是真核生物中高度保守的蛋白质。然而，酵母组蛋白在其序列上更趋不同。特别是裂殖酵母（Schizoschiomyomycespombe）的组蛋白尾部区域，赖氨酸残基较少，使得它们的电荷比高等真核生物的电荷更少。此外，S.粟酒裂殖酵母染色质缺乏连接组蛋白。这些因素如何影响酵母染色质折叠的生化重组结合原子力显微镜分析。核小体阵列的重建表明，S。粟酒裂殖酵母染色质具有类似于重构的人乙酰化染色质的更开放的结构。色葡萄粟酒裂殖酵母核小体阵列形成的纤维比那些在哺乳动物接头组蛋白H1的存在下的人类核小体阵列更细。这样的S.粟酒裂殖酵母纤维与人乙酰化纤维更相似。这些发现表明，核心组蛋白电荷将决定S的内在特征。粟酒裂殖核染色质和影响核小体间的相互作用。
Histones are highly conserved proteins among eukaryotes. However, yeast histones are more divergent in their sequences. In particular, the histone tail regions of the fission yeast, Schizosaccharomyces pombe, have fewer lysine residues, making their charges less positive than those of higher eukaryotes. In addition, the S. pombe chromatin lacks linker histones. How these factors affected yeast chromatin folding was analysed by biochemical reconstitution in combination with atomic force microscopy. Reconstitution of a nucleosome array showed that S. pombe chromatin has a more open structure similar to reconstituted human acetylated chromatin. The S. pombe nucleosomal array formed thinner fibers than those of the human nucleosomal array in the presence of mammalian linker histone H1. Such S. pombe fibers were more comparable to human acetylated fibers. These findings suggest that the core histone charges would determine the intrinsic characteristics of S. pombe chromatin and affect inter-nucleosomal interactions.
H1 连接子组蛋白亚型及其在小鼠中的功能的遗传分析。
影响因子：
--
作者：
Fan,Yuhong;Skoultchi,ArthurI
通讯作者：
Skoultchi,ArthurI
影响因子：
2.9
作者：
Schwarz, PM;Felthauser, A;Hansen, JC
通讯作者：
Hansen, JC
影响因子：
2.9
作者：
HANSEN, JC;AUSIO, J;VANHOLDE, KE
通讯作者：
VANHOLDE, KE