Multi-factors on biodegradation kinetics of polycyclic aromatic hydrocarbons (PAHs) by Sphingomonas sp a bacterial strain isolated from mangrove sediment
Multi-factors on biodegradation kinetics of polycyclic aromatic hydrocarbons (PAHs) by Sphingomonas sp a bacterial strain isolated from mangrove sediment
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DOI：
10.1016/j.marpolbul.2008.03.013
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发表时间：
2008-01-01
影响因子：
5.8
通讯作者：
Tam, Nora F. Y.
中科院分区：
文献类型：
--
作者：
Chen, Jianfin;Wong, M. H.;Tam, Nora F. Y.
受污染沉积物中多环芳烃 (PAH) 的生物降解是一种颇具吸引力的修复技术，其成功取决于生物降解动力学以及 PAH 降解菌株的最佳条件；然而，这方面的信息仍然很少。多因素对鞘氨醇单胞菌污染沉积泥浆中菲（一种三环模型多环芳烃）生物降解的影响。使用正交实验设计（L-16（4（5））形式）对从红树林表面沉积物中分离出的细菌菌株进行了研究。最重要的因素是盐度和接种量，而菲浓度、养分添加和温度的影响并不显着。受污染的红树林沉积物浆液的最佳生物降解条件为30℃、15ppt盐度、100:1的碳/氮比(沉积物中的背景比)和接种量为10(6)最大可能数g(-1)沉积物。在最佳条件下，菲的生物降解可以通过一阶速率模型 C = C(0)e(-kt) 来最好地描述，其中 k（速率常数）等于 0.1185。验证了动力学模型及其在预测鞘氨醇单胞菌生物降解方面的有效性。实验数据证明了在不同菲浓度下的效果。 (C) 2008 Elsevier Ltd. 保留所有权利。
Biodegradation of polycyclic aromatic hydrocarbons (PAHs) in contaminated sediment is an attractive remediation technique and its success depends on biodegradation kinetics, and the optimal condition for the PAH-degrading isolates; however, information on this aspect is still scarce. The effects of multi-factors on biodegradation of phenanthrene, a 3-ring model PAH, in contaminated sediment slurry by Sphingomonas sp. a bacterial strain isolated from surface mangrove sediment, were investigated using the orthogonal experimental design (form L-16(4(5))). The most significant factors were salinity and inoculum size, while the effects of phenanthrene concentrations, nutrient addition and temperatures were insignificant. The optimal biodegradation condition in contaminated mangrove sediment slurry was 30 degrees C, 15 ppt salinity, a carbon/nitrogen ratio of 100:1 (the background ratio in sediment) and an inoculum size of 10(6) Most probable number g(-1) sediment. The phenanthrene biodegradation could be best described by the first order rate model, C = C(0)e(-kt), where k (the rate constant) is equaled to 0.1185, under the optimal condition. The kinetic model was verified and its validity in predicting biodegradation by Sphingomonas sp. at various phenanthrene concentrations was proved by experimental data. (C) 2008 Elsevier Ltd. All rights reserved.