Metalloproteinases, vascular endothelial growth factor, and angiopoietin 1 and 2 in eutopic and ectopic endomnetrium
Metalloproteinases, vascular endothelial growth factor, and angiopoietin 1 and 2 in eutopic and ectopic endomnetrium
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DOI：
10.1016/j.fertnstert.2008.03.079
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发表时间：
2009-06-01
影响因子：
6.7
通讯作者：
Nappi, Carmine
中科院分区：
文献类型：
--
作者：
Di Carlo, Costantino;Bonifacio, Marika;Nappi, Carmine
目的：检测血管内皮生长因子（VEGF）、血管生成素1（Angiopoietin 1，Angiopoietin 2，Angiopoietin 1，Angiopoietin 2（ANGPT 1/ANGPT 2）和基质金属蛋白酶1、2和9（MMP-1，MMP-2，MMP-9）在在位和异位子宫内膜中的表达。设计：实验性回顾性研究。地点：大学医院。患者：30例子宫内膜异位症患者的在位和异位子宫内膜样本和30例健康女性的子宫内膜活检样本。干预：卵巢腺瘤和在位子宫内膜活检。主要结果测量：免疫组化染色评价VEGF、ANGPT 1、ANGPT 2、MMP-1、MMP-2和MMP-9的表达，实时聚合酶链反应分析定量mRNA表达。结论：血管生成因子和金属蛋白酶的过度表达可能是子宫内膜异位症患者子宫内膜的特征性特征，其向腹膜腔异位性病变转化的可能性更大。在位子宫内膜的结构和/或功能差异可能在子宫内膜异位症的发病机制中起作用，继发于子宫内膜细胞向后进入腹腔。这些局部因素是否可以诱导、促进和/或调节这种转化仍有待确定。（Fertil Steril（R）2009;91：2315-23. (C)2009年，美国生殖医学会（American Society for Reproductive Medicine）
Objective: To evaluate the expression of vascular endothelial growth factor (VEGF), angiopoietin 1 and 2 (ANGPT1/ANGPT2), and matrix metalloproteinases 1, 2, and 9 (MMP-1, MMP-2, MMP-9) in eutopic and ectopic endometrium.Design: Experimental retrospective study.Setting: University hospital.Patient(s): Eutopic and ectopic endometrium samples from 30 women with endometriosis and endometrium biopsy samples from 30 healthy women.Intervention(s): Biopsies of ovarian endometriomas and eutopic endometrium.Main Outcome Measure(s): Immunohistochemical staining to evaluate the expression of VEGF, ANGPT1, ANGPT2, MMP-1, MMP-2, and MMP-9, and real-time polymerase chain reaction analysis to quantify mRNA expression.Result(s): Patients with endometriosis had higher levels of angiogenic factors and metalloproteinases in endometriotic cysts than in eutopic endometrium. These substances were also overexpressed in eutopic endometrium of patients with endometriosis when compared with normal controls.Conclusion(s): Overexpression of angiogenic factors and metalloproteinases may be the characteristic feature of endometrium with greater potential to transform into endometriotic lesions in the peritoneal cavity. Structural and/or functional differences of eutopic endometrium could have a role in the pathogenesis of endometriosis secondary to the backward passage of endometrial cells into the peritoneal cavity. Whether these local factors may induce, promote, and/or regulate this transformation remains to be determined. (Fertil Steril (R) 2009;91:2315-23. (C) 2009 by American Society for Reproductive Medicine.)