The effect of silicate ions on proliferation, osteogenic differentiation and cell signalling pathways (WNT and SHH) of bone marrow stromal cells
The effect of silicate ions on proliferation, osteogenic differentiation and cell signalling pathways (WNT and SHH) of bone marrow stromal cells
复制标题
硅酸盐离子对骨髓基质细胞增殖、成骨分化和细胞信号通路（WNT和SHH）的影响
DOI：
10.1039/c2bm00108j
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发表时间：
2013-01-01
影响因子：
6.6
通讯作者：
Xiao, Yin
中科院分区：
文献类型：
--
作者：
Han, Pingping;Wu, Chengtie;Xiao, Yin
硅（Si）是一种微量元素，在人体骨骼生长中起着重要作用。硅已被纳入生物材料用于骨再生，以提高其成骨潜能，无论是在体外还是在体内。然而，对于硅离子如何在骨形成细胞上引起它们的生物学反应，我们所知甚少。本研究旨在通过比较骨髓基质细胞（bone marrow stromal cells, BMSCs）对不同浓度NaCl和Na2SiO3的反应，在考虑和排除Na离子的影响的情况下，探讨Si离子对骨髓基质细胞（bone marrow stromal cells, BMSCs）增殖、分化、骨相关基因表达和细胞信号通路的影响。我们的研究表明，浓度为0.625 mM的Si离子显著增强了骨髓间充质干细胞的增殖、矿化结节的形成、骨相关基因（OCN、OPN和ALP）和骨基质蛋白（ALP和OPN）的表达。此外，0.625 mM的Si离子可以抵消WNT抑制剂（W.I.）小豆蔻素对BMSCs中成骨基因（OPN、OCN和ALP）、WNT和SHH信号通路相关基因表达的影响。这些结果表明，Si离子本身在调节骨髓间充质干细胞的增殖和成骨分化中发挥重要作用，并参与了WNT和SHH信号通路。我们的研究为解释从含硅生物材料中释放的硅离子在所需浓度下获得增强生物活性的可能分子机制提供了证据。
Silicon (Si) is a trace element, which plays an important role in human bone growth. Si has been incorporated into biomaterials for bone regeneration in order to improve their osteogenic potential, both in vitro and in vivo. Little is known, however, as to how Si ions elicit their biological response on bone-forming cells. The aim of this study was to investigate the effect of Si ions on the proliferation, differentiation, bone-related gene expression and cell signalling pathways of bone marrow stromal cells (BMSCs) by comparing the BMSC responses to different concentrations of NaCl and Na2SiO3, while taking into account and excluding the effect of Na ions. Our study showed that Si ions at a concentration of 0.625 mM significantly enhanced the proliferation, mineralization nodule formation, bone-related gene expression (OCN, OPN and ALP) and bone matrix proteins (ALP and OPN) of BMSCs. Furthermore, Si ions at 0.625 mM could counteract the effect of the WNT inhibitor (W.I.) cardamonin on the osteogenic genes expression, (OPN, OCN and ALP), WNT and SHH signalling pathway-related genes in BMSCs. These results suggest that Si ions by themselves play an important role in regulating the proliferation and osteogenic differentiation of BMSCs, with the involvement of WNT and SHH signalling pathways. Our study provides evidence to explain possible molecular mechanisms whereby Si ions released from Si-containing biomaterials can acquire enhanced bioactivity at desired concentration.