Anatomical characteristics and enzymes of the sucrose metabolism and their relationship with latex yield in the rubber tree (Hevea brasiliensis Muell. Arg.)
Anatomical characteristics and enzymes of the sucrose metabolism and their relationship with latex yield in the rubber tree (Hevea brasiliensis Muell. Arg.)
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DOI：
10.1590/s1677-04202006000200002
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发表时间：
2006-06
期刊：
影响因子：
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通讯作者：
A. C. Mesquita;Luiz Edson Oliveira;P. Mazzafera;Nelson Delú-Filho
中科院分区：
文献类型：
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作者：
A. C. Mesquita;Luiz Edson Oliveira;P. Mazzafera;Nelson Delú-Filho
橡胶的生产取决于参与乳胶合成的组织中碳水化合物的可用性。本研究利用3株橡胶树无性系，研究了胶乳的蛋白质谱、转化酶和蔗糖合酶的活性以及树皮的解剖特征，并建立了与胶乳生产的可能关系。研究中使用的无性系RRIM 600、GT 1和Fx2261的乳胶产量从高到低依次不同。虽然存在，但变性电泳获得的乳胶蛋白谱几乎没有差异。中性型转化酶活性高于酸性型，且产量最高的无性系RRIM 600和GT 1的活性均较高。蔗糖合酶也有相同的结果，但这表明转化酶可能在蔗糖在乳胶中的裂解中起更重要的作用。解剖测量结果表明，只有产量最高的克隆RRIM 600的泌乳细胞数、泌乳环数和泌乳细胞直径较高。同时，转化酶活性和解剖测量可能有助于区分高产橡胶树无性系。
Rubber production is dependent on the availability of carbohydrates in the tissues engaged in latex synthesis. In this study three rubber tree clones were used to study the protein profile, the activities of invertase and sucrose synthase in the latex and anatomical characteristics of the bark, as well as to establish a possible relationship with latex production. The clones used in the study, RRIM 600, GT 1 and Fx2261, differed in latex productivity, from high to low in this order. Although present, few differences were observed in the latex protein profile obtained by denaturing electrophoresis. The activity of neutral invertase was higher than the acidic isoform, and both activities were higher in the most productive clones RRIM 600 and GT 1. The same was observed for sucrose synthase but it is suggested that invertase may play a more important role in sucrose cleavage in the latex. The anatomical measurements showed that the number of lactiferous cells and lactiferous rings per mm2, and lactiferous cell diameter were higher only in the most productive clone RRIM 600. Together, invertase activity and anatomical measurements might be useful to distinguish highly productive rubber tree clones.