Mitochondria-related miR-574 reduces sperm ATP by targeting ND5 in aging males
Mitochondria-related miR-574 reduces sperm ATP by targeting ND5 in aging males
复制标题
线粒体相关的 miR-574 通过靶向衰老男性中的 ND5 来减少精子 ATP。
DOI：
10.18632/aging.103141
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发表时间：
2020-05-15
期刊：
影响因子：
5.2
通讯作者：
Yao, Bing
中科院分区：
文献类型：
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作者：
Ma, Jinzhao;Chen, Qiwei;Yao, Bing
近几十年来，夫妇们推迟生育。虽然女性在30多岁时卵子产量明显下降，但父亲年龄对生殖的影响尚不完全清楚。在此，我们观察到许多miRNA，包括miR-574，在老年男性的精子中增加，如高通量测序所示。我们证明了miR-574在两种衰老小鼠模型的精子中上调，并与作为不良预测因子的精子活力低下有关。此外，我们证明了miR-574抑制了GC 2细胞中的线粒体功能并减少了细胞ATP的产生。从机制上讲，我们证明了miR-574通过直接靶向mt-ND 5来调节线粒体功能。我们的研究揭示了miR-574在衰老男性精子功能中的重要作用，为理解精子的衰老过程提供了新的视角。
Couples are delaying childbearing in recent decades. While women experience a notable decrease in oocyte production in their late thirties, the effect of advanced paternal age on reproduction is incompletely understood. Herein, we observed that numerous miRNAs, including miR-574, increased in the sperm of aging males, as indicated by high-throughput sequencing. We demonstrated that miR-574 was upregulated in the sperm of two aging mouse models and was related to inferior sperm motility as an adverse predictor. Moreover, we proved that miR-574 suppressed mitochondrial function and reduced cellular ATP production in GC2 cells. Mechanistically, we demonstrated that miR-574 regulated mitochondrial function by directly targeting mt-ND5. Our study revealed an important role of miR-574 in sperm function in aging males and provided a fresh view to comprehend the aging process in sperm.