Calcium/calmodulin-dependent phosphorylation of vimentin in rat sertoli cells.
Calcium/calmodulin-dependent phosphorylation of vimentin in rat sertoli cells.
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DOI：
10.1073/pnas.80.3.760
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发表时间：
1983-02
影响因子：
11.1
通讯作者：
W. A. Spruill;J. Zysk;L. Tres;A. Kierszenbaum
中科院分区：
文献类型：
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作者：
W. A. Spruill;J. Zysk;L. Tres;A. Kierszenbaum
在大鼠Sertoli细胞原代培养的亚细胞部分中，研究了Ca2+依赖性蛋白磷酸化和钙调蛋白在这一过程中的作用。Sertoli细胞中显著的Ca2+/钙调蛋白依赖性蛋白磷酸化仅限于细胞质部分。使用钙调素抑制剂三氟拉嗪证实了这些作用对钙调素的依赖性。根据以下标准，其中一个Ca2+/钙调素依赖的磷酸化蛋白被确定为中间丝蛋白vimentin: (i)二维聚丙烯酰胺凝胶中的迁移模式，（ii）在Sertoli细胞的不溶性中间丝蛋白提取物中存在的58千道尔顿蛋白的Ca2+/钙调素依赖磷酸化，以及（iii）磷酸化蛋白的肽图谱。这些数据支持Ca2+/钙调蛋白依赖蛋白磷酸化在支持细胞细胞骨架成分的调节中的作用。
Ca2+-dependent protein phosphorylation and the role of calmodulin in this process was investigated in subcellular fractions of primary cultures of rat Sertoli cells. Significant Ca2+/calmodulin-dependent protein phosphorylation in Sertoli cells was restricted to the cytosol fraction. The calmodulin dependence of these effects was confirmed by using the calmodulin inhibitor trifluoperazine. One of the Ca2+/calmodulin-dependent phosphoproteins was identified as the intermediate filament protein vimentin, based on the following criteria: (i) migration pattern in two-dimensional polyacrylamide gels, (ii) Ca2+/calmodulin-dependent phosphorylation of a 58-kilodalton protein present in detergent-insoluble intermediate filament protein extract of Sertoli cells, and (iii) peptide mapping of the phosphoprotein. These data support a role for Ca2+/calmodulin-dependent protein phosphorylation in the modulation of Sertoli cell cytoskeletal components.