Dietary flavonoid kaempferol inhibits glucocorticoid-induced bone loss by promoting osteoblast survival
Dietary flavonoid kaempferol inhibits glucocorticoid-induced bone loss by promoting osteoblast survival
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DOI：
10.1016/j.nut.2017.12.003
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发表时间：
2018-09-01
期刊：
影响因子：
4.4
通讯作者：
Trivedi, Ritu
中科院分区：
文献类型：
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作者：
Adhikary, Sulekha;Choudhary, Dharmendra;Trivedi, Ritu
目的：山萘酚是一种存在于水果和蔬菜中的黄酮类化合物，据报道，它可以逆转去卵巢大鼠的骨质减少。由于山奈酚具有成骨活性，本研究的目的是确定它是否对糖皮质激素（GC）诱导的骨丢失具有有益作用。方法：成年雌性大鼠分为4组作为对照（溶剂;蒸馏水）、甲基强的松龙（MP; 5 mg.kg.d，皮下注射），MP +山奈酚（5 mg.kg.d，口服），和MP +人甲状旁腺1-34（30 μ g/kg，5次/周，皮下注射）并治疗4周。为研究山奈酚对GC诱导的骨折愈合抑制的拮抗作用，对对照组和GC治疗组大鼠进行钻孔损伤实验。口服山奈酚14 d，观察其对损伤部位愈伤组织形成的影响。结果：GC与骨密度降低和骨微结构参数受损有关。在GC诱导的骨质减少条件下消耗山奈酚诱导骨保护作用。此外，在GC动物中观察到山奈酚消耗改善了股骨骨干钻伤部位的骨痂形成。与体内数据一致，山奈酚在体外诱导成骨标志物的表达增加，并拮抗地塞米松对颅骨成骨细胞的凋亡作用。结论：这些结果表明山奈酚减少了GC诱导的骨丢失，促进了骨折部位的骨再生，从而强调了黄酮类化合物对骨骼健康的积极作用。(C)2018爱思唯尔公司All rights reserved.
Objective: Kaempferol, a dietary flavonoid found in fruits and vegetables, has been reported to reverse osteopenic condition in ovariectomized rats. Because kaempferol is endowed with osteogenic activity, the aim of this study was to determine whether it has a beneficial effect on glucocorticoid (GC)-induced bone loss.Methods: Adult female rats were divided into four groups as control (vehicle; distilled water), methylprednisolone (MP; 5 mg.kg.d, subcutaneously), MP + kaempferol (5 mg.kg.d, oral), and MP + human parathyroid 1-34 (30 mu g/kg, 5 times/wk, subcutaneously) and treated for 4 wk. To study the antagonizing effect of kaempferol on GC-induced inhibition of fracture healing, drill-hole injury was performed on control and GC-treated rats. An oral dose of kaempferol was given for 14 d to observe the effect on callus formation at the site of injury. After treatment, bones were collected for further analysis.Results: GC was associated with a decreased bone mineral density and impaired bone microarchitecture parameters. Consumption of kaempferol induced bone-sparing effects in GC-induced osteopenic condition. Additionally, improved callus formation at site of drill injury in femur diaphysis was observed with kaempferol consumption in animals on GC. Consistent with the in vivo data, kaempferol elicited a higher expression of osteogenic markers in vitro and antagonized the apoptotic effect of dexamethasone on calvarial osteoblasts.Conclusion: These results suggested that kaempferol reduced GC-induced bone loss and enhanced bone regeneration at fractured site, thus emphasizing the positive role of flavonoids on bone health. (C) 2018 Elsevier Inc. All rights reserved.