Direct determination of carbohydrates, amino acids, and antibiotics by microchip electrophoresis with pulsed amperometric detection
Direct determination of carbohydrates, amino acids, and antibiotics by microchip electrophoresis with pulsed amperometric detection
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DOI：
10.1021/ac034440v
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发表时间：
2003-09-15
影响因子：
7.4
通讯作者：
Henry, CS
中科院分区：
文献类型：
--
作者：
García, CD;Henry, CS
描述了用脉冲安培检测（PAD）的电泳微芯片分离和检测未气化的碳水化合物、氨基酸和含硫抗生素。本报告还介绍了一种新的芯片配置的微芯片电泳与PAD的发展。该结构由一层聚（二甲基硅氧烷）组成，其中包含微流体通道、储层和金微线，密封到第二层聚（二甲基硅氧烷）。示出了碳水化合物、氨基酸和含硫抗生素的分离实例。研究了分离和进样电位、缓冲液pH和组成、进样时间和PAD参数对分离和检测的影响。青霉素和氨苄西林的检出限为6 fmol (5 muM)，组氨酸的检出限为455 fmol （350 muM）。
The separation and detection of underivatized carbohydrates, amino acids, and sulfur-containing antibiotics in an electrophoretic microchip with pulsed amperometric detection (PAD) is described. This report also describes the development of a new chip configuration for microchip electrophoresis with PAD. The configuration consists of a layer of poly(dimethylsiloxane) that contains the microfluidic channels, reservoirs, and a gold microwire, sealed to a second layer of poly(dimethylsiloxane). Example separations of carbohydrates, amino acids, and sulfur-containing antibiotics are shown. The effect of the separation and injection potentials, buffer pH and composition, injection time, and PAD parameters were studied in an effort to optimize separations and detection. Detection limits ranging from 6 fmol (5 muM) for penicillin and ampicillin to 455 fmol (350 muM) for histidine were obtained.