Adipogenic Differentiation of Human Mesenchymal Stem Cells
Adipogenic Differentiation of Human Mesenchymal Stem Cells
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DOI：
10.1007/978-1-60761-999-4_19
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发表时间：
2011-01-01
期刊：
影响因子：
--
通讯作者：
Zachar, Vladimir
中科院分区：
文献类型：
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作者：
Fink, Trine;Zachar, Vladimir
间充质干细胞具有分化为包括脂肪细胞在内的多种细胞类型的能力。脂肪细胞表型的特征在于脂滴的细胞内积累以及脂肪细胞特异性基因的转录。本文详细介绍了骨髓和脂肪组织来源的干细胞成脂诱导的基本方案，以及通过实时RT-PCR对脂质积累进行染色和对PPAR-gamma和aP 2进行转录分析的方案。
Mesenchymal stem cells have the capability to differentiate into a number of cell types including adipocytes. The adipocytic phenotype is characterized by intracellular accumulation of lipid droplets as well as transcription of adipocyte-specific genes. This paper details a basic protocol for adipogenic induction of bone marrow and adipose tissue-derived stem cells, as well as protocols for staining lipid accumulation and the transcriptional analysis of PPAR-gamma and aP2 by real-time RT-PCR.