Cyclic strain promotes shuttling of PYK2/Hic-5 complex from focal contacts in osteoblast-like cells
Cyclic strain promotes shuttling of PYK2/Hic-5 complex from focal contacts in osteoblast-like cells
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DOI：
10.1016/j.bbrc.2006.02.162
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发表时间：
2006-05-05
影响因子：
3.1
通讯作者：
Lafage-Proust, MH
中科院分区：
文献类型：
--
作者：
Guignandon, A;Boutahar, N;Lafage-Proust, MH
我们发现，周期应变（CS）的成骨细胞诱导的两个同源酪氨酸激酶FAK和PYK 2的酪氨酸磷酸化，和两个同源衔接蛋白桩蛋白和HIC 5，具有相似的动力学。免疫染色显示，所有四种蛋白质都定位于对照组的焦点接触。相反，CS后观察到的亚细胞定位的动力学不同。而FAK和桩蛋白仍在局灶接触，Hic-5和PYK 2易位腹侧局灶接触外，早在30分钟后CS和被隔离的细胞骨架。免疫共沉淀结果显示，PYK 2/Hic-5和PYK 2/ FAK的结合随时间延长而增强，而桩蛋白和Hic-5的结合随时间延长而减弱。总之，这些结果表明，CS调节成骨细胞中的焦点接触活性，通过调节含PYK 2的复合物，特别是通过穿梭出焦点接触的衔接子Hic-5和有利于FAK在接触内的锚定。(c)2006爱思唯尔公司All rights reserved.
We showed that cyclic strain (CS) of osteoblastic cells induced tyrosine phosphorylation of two homologous tyrosine kinases FAK and PYK2, and of two homologous adaptor proteins paxillin and Hic5, with similar kinetics. Immunostaining showed that all four proteins were localized to focal contacts in controls. In contrast, the dynamics of their subcellular localization observed after CS differed. While FAK and paxillin remained at the focal contact, Hic-5 and PYK2 translocated outside ventral focal contacts as early as 30 min after CS and were sequestered by the cytoskeleton. Co-immunoprecipitation showed that the association of PYK2/Hic-5 and PYK2/ FAK increased with time after strain while that of paxillin and Hic-5 decreased. Altogether these results suggested that CS regulates focal contact activity in osteoblasts by modulating PYK2-containing complexes in particular by shuttling out of the focal contact the adaptor Hic-5 and favoring the anchorage of FAK within contacts. (c) 2006 Elsevier Inc. All rights reserved.