EFFECTS OF MOT GENE-EXPRESSION ON THE STRUCTURE OF THE FLAGELLAR MOTOR
EFFECTS OF MOT GENE-EXPRESSION ON THE STRUCTURE OF THE FLAGELLAR MOTOR
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DOI：
10.1016/0022-2836(88)90287-2
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发表时间：
1988-08-05
影响因子：
5.6
通讯作者：
REESE, TS
中科院分区：
文献类型：
--
作者：
KHAN, S;DAPICE, M;REESE, TS
直接冷冻程序使我们能够通过冷冻断裂电子显微镜观察到围毛鞭毛细菌细胞质膜中独特的膜内颗粒环结构。这些结构被确定为鞭毛马达组件，因为它们的分布相匹配的鞭毛，因为它们是不存在的大肠杆菌的非鞭毛突变体。革兰氏阳性链球菌和革兰氏阴性E.杆菌在大肠大肠杆菌，一个无功能的摩卡操纵子产生的鞭毛，但不动的细胞缺乏颗粒环。同时引入motA和motB基因，导致运动性和环结构的恢复，但单独的基因是不够的。伴随的环和运动性的损失是一致的，在鞭毛电机中具有核心作用的环颗粒。
Direct freezing procedures have enabled us to visualize distinctive intramembrane particle ring structures in the cytoplasmic membranes of peritrichously flagellated bacteria by means of freeze-fracture electron microscopy. These structures were identified as flagellar motor components because their distribution matched that of flagella, and because they were absent in non-flagellated mutants of Escherichia coli. Particle rings were present in both the Gram-positive Streptococcus and the Gram-negative E. coli. In E. coli, a nonfunctional mocha operon produced flagellated but immotile cells lacking the particle rings. Simultaneous introduction of the motA and motB genes, led to recovery of both motility and the ring structures but neither gene alone was sufficient. The concomitant loss of the rings and motility is consistent with the ring particles having a central role in the flagellar motor.