Silver nanoparticle-treated filter paper as a highly sensitive surface-enhanced Raman scattering (SERS) substrate for detection of tyrosine in aqueous solution
Silver nanoparticle-treated filter paper as a highly sensitive surface-enhanced Raman scattering (SERS) substrate for detection of tyrosine in aqueous solution
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DOI：
10.1016/j.aca.2011.10.013
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发表时间：
2011-12-05
影响因子：
6.2
通讯作者：
Yang, Jyisy
中科院分区：
文献类型：
--
作者：
Cheng, Min-Liang;Tsai, Bo-Chan;Yang, Jyisy
在这项工作中，高灵敏度的表面增强Sers散射基板上的银纳米粒子的沉积在市售滤纸的基础上，并用于克服在分析水溶液样品中发现的问题。为了制备掺杂银纳米颗粒（AgNP）的滤光片基底，使用银镜反应。系统地优化了底物制备工艺。对银镜反应的滤纸预处理、反应时间、反应温度、试剂浓度等进行了研究。通过场发射扫描电子显微镜（FE-SEM）表征所得基底的形貌，并通过对硝基硫酚（pNTP）探测与Sers信号相关。经不同预处理的滤纸上银纳米粒子的大小和分布不同。当滤纸在AgNPs生长之前用氨溶液预处理时，发现性能最好。基于SEM图像，所得的AgNP具有大致球形的形状，具有高度的均匀性。与玻璃基底相比，镀银滤纸基底提供了更高的Sers信号，并且重现性显著提高。基于统计分析，基质与基质和点与点的相对标准偏差均小于8%，基质的增强因子通常高于107。利用该Sers基底选择性地检测了水溶液中的酪氨酸。结果表明，基于过滤器的Sers基底非常适合检测酪氨酸。与玻璃基Sers基底相比，在酪氨酸检测中观察到50倍以上的Sers信号。线性范围可达100 μ M，检测限为625 nM（SN-1 = 3）。(C)2011爱思唯尔有限公司版权所有。
Highly sensitive SERS substrates based on deposition of silver nanoparticles on commercially available filter paper were prepared in this work, and used to overcome problems found in analyses of aqueous samples. To prepare silver nanoparticle- (AgNP) doped filter substrates, a silver mirror reaction was used. The procedures for substrate preparation were systematically optimized. Pretreatment of filter paper, reaction time, temperature, and concentration of reagents for silver mirror reactions were studied. The morphologies of the resulting substrates were characterized by field-emission scanning electron microscopy (FE-SEM) and correlated with the SERS signals by probing with p-nitrothiophenol (pNTP). Filter papers with different pretreatments were found to have different sizes and distributions of AgNPs. The best performance was found when filter paper was pre-treated with ammonia solution before growth of AgNPs. Based on the SEM images, the resulting AgNPs had roughly spherical shape with a high degree of uniformity. The silver-coated filter paper substrates provide much higher SERS signals compared to glass substrates and the reproducibility was improved significantly. Based on statistical analyses, the relative standard deviations for substrate-to-substrate and spot-to-spot were both were less than 8% and the enhancement factors for the substrates were, in general, higher than 107. The SERS substrates were used to selectively detect tyrosine in aqueous solution. Results indicate that filter-based SERS substrates are highly suited to detection of tyrosine. Compared to glass-based SERS substrates, 50 times more SERS signal was observed in detection of tyrosine. The linear range can be up to 100 mu M with a detection limit of 625 nM (S N-1 = 3). (C) 2011 Elsevier B.V. All rights reserved.