Protective immunity is induced by a Borrelia burgdorferi mutant that lacks OspA and OspB.
Protective immunity is induced by a Borrelia burgdorferi mutant that lacks OspA and OspB.
复制标题
保护性免疫是由缺乏 OspA 和 OspB 的伯氏疏螺旋体突变体诱导的。
DOI：
10.1128/iai.61.12.5115-5122.1993
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发表时间：
1993
影响因子：
3.1
通讯作者：
Johnson,RC
中科院分区：
文献类型：
--
作者：
Hughes,CA;Engstrom,SM;Coleman,LA;Kodner,CB;Johnson,RC
通过在5℃下的长期贮藏，选择了一株强毒伯氏疏螺旋体297突变体，发现该突变体缺乏编码外表面蛋白A （OspA）和OspB的质粒。除了OspA和OspB蛋白缺失外，该突变体还缺少两种脂蛋白，分别为20和7.5 kDa，这在野生型中观察到。由于突变体不能从注射了突变体的仓鼠的组织或血液中恢复，因此确定该突变体不具有传染性。接种了非传染性突变体297的仓鼠完全免受毒性野生型297螺旋体的攻击。接种突变型297的仓鼠攻毒前血清缺乏对OspA和OspB的抗体，而接种毒力野生型297或无毒型297的仓鼠则表现出对这些蛋白的抗体。接种毒力野生型297或突变型297的仓鼠可引发OspC抗体和39-kDa蛋白（P39）抗体，而接种无毒型297的仓鼠则缺乏这些抗体。这些结果表明OspC和/或P39对保护性免疫反应的发展很重要。对该突变体的研究可能阐明莱姆病疫苗研制的重要因素。
A mutant of virulent Borrelia burgdorferi 297 was apparently selected for by long-term storage at 5 degrees C. This mutant was found to lack the plasmid which encodes outer surface protein A (OspA) and OspB. In addition to the loss of the OspA and OspB proteins, the mutant lacked two lipoproteins, of 20 and 7.5 kDa, that were observed in the wild type. Since the mutant was not recovered from the tissues or blood of hamsters injected with the mutant, the mutant was determined to be noninfectious. Hamsters vaccinated with noninfectious mutant 297 were protected completely from challenge with virulent wild-type 297 spirochetes. Prechallenge sera from hamsters vaccinated with mutant 297 lacked antibodies to OspA and OspB, while those from hamsters vaccinated with virulent wild-type 297 or avirulent 297 exhibited antibodies to these proteins. Hamsters vaccinated with virulent wild-type 297 or mutant 297 elicited antibodies to OspC and a 39-kDa protein (P39), whereas hamsters vaccinated with avirulent 297 lacked these antibodies. These results suggest that OspC and/or P39 are important for the development of a protective immune response. Study of this mutant may elucidate factors important to the development of a Lyme disease vaccine.