MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures
MAO, COMT, and GABA‐T Activities in Primary Astroglial Cultures
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原代星形胶质细胞培养中的 MAO、COMT 和 GABA-T 活动
DOI：
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发表时间：
1983
影响因子：
4.7
通讯作者：
Å. Sellström
中科院分区：
文献类型：
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作者：
E. Hansson;Å. Sellström
摘要：新生大鼠大脑半球培养物中含有单胺氧化酶（MAO）、儿茶酚- O -甲基转移酶（COMT）和α -氨基丁酸- γ -酮戊二酸转氨酶（GABA - T）。COMT活性在培养物中高于成年大鼠大脑半球。在培养物和大鼠大脑半球中，MAO活性是相当的。这两种酶的活性在培养物和大鼠大脑半球中随着年龄的增长而增加。在培养中，通过去除胎牛血清和添加0.1 mM二丁基环AMP （dB - cAMP）进一步增强了活性。然而，GABA‐T活性在培养物中低于成年大鼠的大脑半球。在产后成熟过程中，大脑的活动增加。在培养物中，无论是在生长过程中，还是在去除胎牛血清并添加dB - cAMP后，都没有观察到酶活性的变化。
Abstract: Cultures from cerebral heimspheres of newborn rats contain the enzymes monoamine oxidase (MAO), catechol‐O‐methyltransferase (COMT), and α‐aminobutyric acid γ‐ketoglutarate transaminase (GABA‐T). The COMT activity was higher in the cultures than in adult rat cerebral hemispheres. The MAO activity was comparable in the cultures and in the rat cerebral hemispheres. The activities of both these enzymes increased with age in the cultures and in the rat brain hemispheres. In the culture the activities were further potentiated by removal of fetal calf serum and addition of 0.1 mM dibutyryl‐cyclic AMP (dB‐cAMP). GABA‐T activity was, however, lower in the cultures than in the adult rat brain hemispheres. The activity increased in brain during postnatal maturation. No changes in the enzyme activity were observed in the cultures, either during growth or after removal of fetal calf serum and addition of dB‐cAMP.