Extracellular terpenoid hydrocarbon extraction and quantitation from the green microalgae Botryococcus braunii var. Showa
Extracellular terpenoid hydrocarbon extraction and quantitation from the green microalgae Botryococcus braunii var. Showa
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DOI：
10.1016/j.biortech.2009.11.043
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发表时间：
2010-04-01
影响因子：
11.4
通讯作者：
Melis, Anastasios
中科院分区：
文献类型：
--
作者：
Eroglu, Ela;Melis, Anastasios
采用机械分级法和水/有机两相分配法对布朗葡萄球菌（Botryococcus braunii var.）昭和设计了一种直接分光光度法定量葡萄球菌和相关的类胡萝卜素烃提取这种方法。在水中用玻璃珠涡旋微菌落，然后通过浮力密度平衡将烃与生物质分离，或者在庚烷作为溶剂的存在下，然后通过庚烷溶解的烃（上层相）与生物质（下层水相）的水/有机两相分离，实现细胞外葡萄球菌属烃与葡萄球菌属的分离。上庚烷相的光谱分析揭示了存在两种不同的化合物，一种在UV-C中吸收，归因于葡萄球菌，另一种在光谱的蓝色区域，归因于类胡萝卜素。在庚烷中，在190 nm处（λ = 90 +/- 5 mM（-1）cm（-1））和450 nm处（λ = 165 +/- 5 mM（-1）cm-1），对三萜类和类胡萝卜素的吸光度进行了比消光系数测定。这使得应用直接分光光度法定量水或庚烷提取的葡萄球菌和类胡萝卜素。B。布劳尼湾昭和组成型积累类似于30%的干生物质作为可提取的（细胞外）葡萄球菌，和类似于0.2%的干生物质的形式的类胡萝卜素。进一步证明了葡萄藻属生物质的热处理显著加速了提取过程的速率和产率。在这项工作中的进展作为一个循环葡萄球菌生长的基础，无毒提取细胞外烃，并返回的烃耗尽的生物质的生长条件，进一步的产品生成。(C)2009爱思唯尔有限公司保留所有权利。
Mechanical fractionation and aqueous or aqueous/organic two-phase partition approaches were applied for extraction and separation of extracellular terpenoid hydrocarbons from Botryococcus braunii var. Showa. A direct spectrophotometric method was devised for the quantitation of botryococcene and associated carotenoid hydrocarbons extracted by this method. Separation of extracellular botryococcene hydrocarbons from the Botryococcus was achieved upon vortexing of the micro-colonies with glass beads, either in water followed by buoyant density equilibrium to separate hydrocarbons from biomass, or in the presence of heptane as a solvent, followed by aqueous/organic two-phase separation of the heptane-solubilized hydrocarbons (upper phase) from the biomass (lower aqueous phase). Spectral analysis of the upper heptane phase revealed the presence of two distinct compounds, one absorbing in the UV-C, attributed to botryococcene(s), the other in the blue region of the spectrum, attributed to a carotenoid. Specific extinction coefficients were developed for the absorbance of triterpenes at 190 nm (epsilon = 90 +/- 5 mM(-1) cm(-1)) and carotenoids at 450 nm (epsilon = 165 +/- 5 mM(-1) cm-1) in heptane. This enabled application of a direct spectrophotometric method for the quantitation of water- or heptane-extractable botryococcenes and carotenoids. B. braunii var. Showa constitutively accumulates similar to 30% of the dry biomass as extractable (extracellular) botryococcenes, and similar to 0.2% of the dry biomass in the form of a carotenoid. It was further demonstrated that heat-treatment of the Botryococcus biomass substantially accelerates the rate and yield of the extraction process. Advances in this work serve as foundation for a cyclic Botryococcus growth, non-toxic extraction of extracellular hydrocarbons, and return of the hydrocarbon-depleted biomass to growth conditions for further product generation. (C) 2009 Elsevier Ltd. All rights reserved.