Determination of biogenic amines in wines by ion-pair liquid chromatography and post-column derivatization with 1,2-naphthoquinone-4-sulphonate
Determination of biogenic amines in wines by ion-pair liquid chromatography and post-column derivatization with 1,2-naphthoquinone-4-sulphonate
复制标题
DOI：
10.1016/j.chroma.2006.05.090
复制
发表时间：
2006-10-13
影响因子：
4.1
通讯作者：
Saurina, Javier
中科院分区：
文献类型：
--
作者：
Hlabangana, Leah;Hernandez-Cassou, Santiago;Saurina, Javier
建立了葡萄酒中生物胺的柱后衍生液相色谱测定方法。该方法是基于分离胺的离子对色谱使用庚烷磺酸钠（SHS）和在线标记的分析物与1，2-萘醌-4-磺酸。通过析因设计和多目标决策，考虑了影响分离的主要因素（乙腈和SHS浓度），优化了洗脱梯度。使用红葡萄酒样品建立了品质因数。方法的检出限为0.2 ~ 3 mg·L ~（-1），峰面积重复性为1.6 ~ 4.6%，保留时间重复性小于1.2%。回收率在92%和108%之间，证明了该方法测定商业红葡萄酒中乙醇胺、乙胺、组胺和酪胺的准确性。所提出的方法已被应用到不同的西班牙地区的葡萄酒的分析。(c)2006 Elsevier B. V.保留所有权利。
A liquid chromatographic method with post-column derivatization for the determination of biogenic amines in wines is proposed. The method is based on the separation of amines by ion-pair chromatography using sodium heptanesulfonate (SHS) and on-line labeling of analytes with 1,2-naphthoquinone-4-sulfonate. The principal factors influencing the separation (acetonitrile and SHS concentration) have been considered for the optimization of the elution gradient through factorial design and multicriteria decision-making. Figures of merit have been established using red wine samples. Detection limits range from 0.2 to 3 mg L-1, the peak area run-to-run repeatability from 1.6 to 4.6% and the retention time repeatability lower than 1.2%. Recoveries ranging from 92 and 108% prove the accuracy of the method for determining ethanolamine, ethylamine, histamine and tyramine in commercial red wines. The proposed method has been applied to the analysis of wines from different Spanish regions. (c) 2006 Elsevier B.V. All rights reserved.