Studies on cultured and uncultured microbiota of wild Culex quinquefasciatus mosquito midgut based on 16s ribosomal RNA gene analysis
Studies on cultured and uncultured microbiota of wild Culex quinquefasciatus mosquito midgut based on 16s ribosomal RNA gene analysis
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DOI：
10.4269/ajtmh.2004.70.597
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发表时间：
2004-06-01
影响因子：
3.3
通讯作者：
Shouche, YS
中科院分区：
文献类型：
--
作者：
Pidiyar, VJ;Jangid, K;Shouche, YS
为了解野生致倦库蚊中肠微生物多样性及转基因蚊虫的候选细菌，采用常规培养技术和16 SrRNA基因文库分析方法，对致倦库蚊中肠微生物区系进行了研究。可培养的微生物群被鉴定为琼氏不动杆菌（Acinetobacter junii），Ac.醋酸钙单胞菌、库利氏气单胞菌、苏云金芽孢杆菌、氧化微杆菌、成团泛菌、铜绿假单胞菌、表皮葡萄球菌、嗜麦芽寡养单胞菌和来自宿主果蝇的未鉴定的细菌。16 SrRNA基因文库中未鉴定和未培养的细菌占46%，不动杆菌属占41%，乳球菌属（Lactococcus spp.）计算的150个克隆的覆盖率为83.3%。因此，下一个克隆序列落入新的操作分类单位（尚未观察到）的概率为16.7%。大多数培养的菌株和16 S rRNA基因文库克隆属于γ-变形菌纲。此前有报道称，大多数细菌栖息在不同蚊子物种的中肠中。因此，本研究的结果表明，不同的蚊子物种具有共同的微生物群代表，这些微生物群可能是遗传操作的潜在候选者，以控制宿主的疾病传播能力。
To describe the midgut microbial diversity and the candidate bacteria for the genetic manipulation for the generation of transgenic mosquitoes refractory to transmission of diseases, the microbiota of wild Culex quinquefasciatus mosquito midgut was studied using a conventional culture technique and analysis of a 16S ribosomal RNA (rRNA) gene sequence library. The culturable microbiota was identified as Acinetobacter junii, Ac. calcoaceticus, Aeromonas culicicola, Bacillus thuringiensis, Microbacterium oxydans, Pantoea agglomerans, Pseudomonas aeruginosa, Staphylococcus epidermidis, Stenotrophomonas maltophila and an unidentified bacterium from the host Drosophila paulistorium. The 16S rRNA gene library was composed of 46% unidentified and uncultured bacteria, 41% Acinetobacter spp., and 13% Lactococcus spp. The coverage calculated for the 150 clones was 83.3%. Thus, the probability of the next cloned sequence falling in a novel operational taxonomic unit (not yet observed) was 16.7%. The majority of the cultured isolates and the 16S rRNA gene library clones belonged to the gamma-proteobacteria class. Most of the bacteria have been previously reported to inhabit the midgut of different mosquito species. Therefore, the results of this study indicate that different mosquito species harbor common representatives of the microbiota that may be the potential candidates for genetic manipulation to control the disease transmission capabilities of the host.