Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9
Improving pre-harvest sprouting resistance in rice by editing OsABA8ox using CRISPR/Cas9
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DOI：
10.1007/s00299-022-02917-3
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发表时间：
2022-08
影响因子：
6.2
通讯作者：
Kaixin Fu;Weihan Song;Cheng Chen;C. Mou;Yunshuai Huang;Fulin Zhang;Qixian Hao;Ping Wang;Tengfei Ma;Yaping Chen;Ziyan Zhu;Min Zhang;Qikai Tong;Xi Liu;Ling Jiang;J. Wan
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文献类型：
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作者：
Kaixin Fu;Weihan Song;Cheng Chen;C. Mou;Yunshuai Huang;Fulin Zhang;Qixian Hao;Ping Wang;Tengfei Ma;Yaping Chen;Ziyan Zhu;Min Zhang;Qikai Tong;Xi Liu;Ling Jiang;J. Wan
Knock outOsABA 8 ox有助于提高收获前的抗穗发芽能力，并且不影响水稻产量。收获前发芽是指作物种子在收获前发芽的现象，它会降低水稻的产量和品质。脱落酸（阿坝）是促进种子休眠的植物激素之一。ABA 8 '羟化酶是植物体内催化阿坝的主要酶。水稻中编码ABA 8 '羟化酶的基因有OsABA 8 ox 1、OsABA 8 ox 2和OsABA 8 ox 3。本研究利用CRISPR/Cas9基因编辑技术对宁粳6号中的这三个基因进行靶向敲除，发现敲除后的转基因株系种子休眠性均显著增强，且对产量没有影响。通过一系列的定量实验，我们认为OsABA 8 ox基因敲除后，内源阿坝水平升高会影响阿坝信号，从而抑制种子的物质和能量代谢，最终导致种子休眠加剧。
Key messageKnock outOsABA8oxhelps improve pre-harvest spouting resistance and do not affect rice yield. Pre-harvest sprouting(PHS) is a phenomenon that the seeds of crops germinate preharvest, which reduces the yield and quality of rice. Abscisic acid(ABA) is one of the phytohormones that promotes seed dormancy. ABA8’ hydroxylase is the main enzyme that can catabolism ABA in plant. There are three genes that encode ABA8’ hydroxylase in rice, namedOsABA8ox1,OsABA8ox2andOsABA8ox3. In this study, we use CRISPR/Cas9 gene editing technology to target these three genes in Ningjing6 and find that the knockout transgenic lines are all significantly strengthen in seed dormancy and have no effect on the yield. By a series of quantitative experiments, we consider that after knock outOsABA8ox, the high endogenous ABA level will influence the ABA signal which suppress the substantial and energy metabolism in the seeds, and finally led to higher dormancy.