Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning
Long-Term Potable Effects of Alkalescent Mineral Water on Intestinal Microbiota Shift and Physical Conditioning
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DOI：
10.1155/2019/2710587
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发表时间：
2019-11-19
影响因子：
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通讯作者：
Inomata, Masafumi
中科院分区：
文献类型：
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作者：
Yahiro, Takaaki;Hara, Takao;Inomata, Masafumi
背景。位于日本九州岛西北部的日田盆地的弱碱性（pH 8.3）矿泉水（AMW）因其含有高浓度硅酸、钠、钾和碳酸氢盐等成分的独特品质而受到认可。对 AMW 摄入量的生物效应进行了评估，特别关注其通过调节肠道微生物群的“抗肥胖”特性。方法。两组 C57BL6/J 小鼠（8 周龄雄性）维持标准饮食和自来水（对照：TWC 组）或 AMW（AMW 组）6 个月，并对以下输出进行定量：（1）食物和水摄入量，（2）体重（每周），（3）CT 扫描体脂测量（每月），（4）血清生化值（TG、ALT、AST 和 ALP），以及（5）脂肪组织中的 UCP-1 mRNA（终点）。采用相同方法饲养两组ICR小鼠（7周龄雄性），并在第0、1、3和6个月时收集其粪便，利用16S-rRNA宏基因组测序分析此时肠道微生物群的数量率。结果。在所有抗肥胖测试项目中，即使每周饮食摄入量增加（p=0.012），AMW组的体重增加比率（p=1.21E-10）和内脏脂肪积累比率（p=0.029）均显着降低。其他标准包括水摄入量（p=0.727）、总量（p=0.1602）和皮下脂肪积累（p=0.052）均在误差范围内，并且AMW组中UCP-1基因表达水平（p=0.171）比TWC组高3.89倍。在 8 个主要肠道细菌家族中，乳杆菌科（增加，p=0.029）和梭菌科（减少，p=0.029）在整个种群中表现出显着变化。结论。我们观察到（1）体重增加率（平均-1.86％，最高-3.3％）显着降低，（2）内脏脂肪积累率（平均-4.30％，最高-9.1％）和（3）肠道微生物群的变化。所有这些后果都可以支持 AMW 的“健康益处”功能。
Background. An alkalescent (pH 8.3) mineral water (AMW) of Hita basin, located in the northwestern part of Kyushu island in Japan, has been recognized for the unique quality of ingredients including highly concentrated silicic acid, sodium, potassium, and hydrogen carbonate. The biological effects of AMW intake were evaluated with a particular focus on its "antiobesity" properties through its modulation of the gut microbiota population. Methods. Two groups of C57BL6/J mice (8-week-old male) were maintained with a standard diet and tap water (control: TWC group) or AMW (AMW group) for 6 months and the following outputs were quantitated: (1) food and water intake, (2) body weight (weekly), (3) body fat measurements by CT scan (monthly), (4) sera biochemical values (TG, ALT, AST, and ALP), and (5) UCP-1 mRNA in fat tissues (terminal point). Two groups of ICR mice (7-week-old male) were maintained with the same method and their feces were collected at the 0, 1st, 3rd, and 6th month at which time the population rates of gut microbiota were quantitated using metagenomic sequencing analysis of 16S-rRNA. Results. Among all antiobesity testing items, even though a weekly dietary consumption was increased (p=0.012), both ratios of weight gain (p=1.21E-10) and visceral fat accumulation (p=0.029) were significantly reduced in the AMW group. Other criteria including water intake (p=0.727), the amounts of total (p=0.1602), and subcutaneous fat accumulation (p=0.052) were within the margin of error and UCP-1 gene expression level (p=0.171) in the AMW group was 3.89-fold higher than that of TWC. Among 8 major gut bacteria families, Lactobacillaceae (increased, p=0.029) and Clostridiaceae (decreased, p=0.029) showed significant shift in the whole population. Conclusion. We observed significantly reduced (1) weight gaining ratio (average -1.86%, up to -3.3%), (2) visceral fat accumulation ratio (average -4.30%, up to -9.1%), and (3) changes in gut microbiota population. All these consequences could support the "health benefit" functionality of AMW.