Studies on sex-organ development. Isolation and characterization of an oestrogen receptor from chick Müllerian duct.
Studies on sex-organ development. Isolation and characterization of an oestrogen receptor from chick Müllerian duct.
复制标题
鸡苗勒管雌激素受体的分离和表征研究。
作者：
C. Teng;C. Teng
在15日龄雌性鸡胚的左侧米勒管中观察到一种雌二醇结合大分子。胚胎受体以高亲和力和低容量结合雌二醇，其解离常数（Kd）为3.2×10⁻⁹摩尔/升，左侧米勒管中每10⁶个细胞的最大结合位点数量为5.45飞摩尔。该受体本质上是蛋白质，这从其对蛋白水解的敏感性可以看出；此外，它具有器官特异性和类固醇特异性。根据甘油梯度分析判断，胞质溶胶中的激素受体以8S和4.5S形式存在，在0.5摩尔/升氯化钾存在的情况下，8S形式可解离为4.5S形式。可从细胞核中提取出4.5 - 6S的受体。在生理盐条件下，胚胎受体与DNA - 纤维素结合，当盐浓度增加到0.5摩尔/升氯化钾时可被洗脱。等电聚焦测定表明，8S受体的等电点为5.8，4.5S受体的等电点为6.9。
An oestradiol-binding macromolecule was observed in the left Müllerian duct of the 15-day female chick embryo. The embryonic receptor binds oestradiol with a high affinity and low capacity, having a Kd of 3.2 X 10(-9)M and a maximal number of sites of 5.45 fmol/10(6) cells in the left Müllerian duct. The receptor is protein in nature, as suggested by its susceptibility to proteolysis; in addition, it is organ- and steroid-specific. Judging by glycerol-gradient analysis, the hormone receptors in the cytosol are present in 8S and 4.5S forms, and the 8S form could be dissociated into a 4.5S form in the presence of 0.5M-KCl. A 4.5-6S receptor could be extracted from the nuclei. Under physiological salt conditions, the embryonic receptors bind to DNA-cellulose and can be eluted when the salt concentration is increased to 0.5M-KCl. Determination by isoelectric focusing indicates that the isoelectric point is 5.8 for the 8S and 6.9 for the 4.5S receptor.