Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide.
Immobilization of a carbon nanomaterial-based localized drug-release system using a bispecific material-binding peptide.
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DOI：
10.2147/ijn.s155913
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发表时间：
2018
影响因子：
8
通讯作者：
Shiba K
中科院分区：
文献类型：
--
作者：
Kokubun K;Matsumura S;Yudasaka M;Iijima S;Shiba K
无机材料被广泛用于医疗器械，如人造心脏，血管和关节，支架，以及作为药物输送系统的纳米载体。碳纳米材料由于其生物惰性和容纳分子的能力而特别感兴趣。已经提出了几种尝试，其中碳纳米材料被用作全身递送药物的纳米载体。我们开发了一种药物递送系统，其中使用材料结合肽将氧化单壁碳纳米角（oxSWNHs）固定在钛（Ti）表面上，以实现局部药物递送。为此目的，我们利用了包含Ti结合肽和SWNH结合肽的核心序列的双特异性肽适体以在Ti上结合oxSWNH。扫描电子显微镜被用来确认存在的oxSWNH吸附到Ti表面上，和石英晶体微量天平被用来评估oxSWNH吸附过程中的结合过程。修饰oxSWNHS的Ti基底对细胞无毒，并在持续时间内释放具有生物活性的地塞米松。这种oxSWNH固定化系统可用于修饰植入物中的Ti表面，并装载刺激骨生成和骨再生的药物。
Inorganic materials are widely used in medical devices, such as artificial hearts, vessels, and joints, in stents, and as nanocarriers for drug-delivery systems. Carbon nanomaterials are of particular interest due to their biological inertness and their capability to accommodate molecules. Several attempts have been proposed, in which carbon nanomaterials are used as nanocarriers for the systemic delivery of drugs. We developed a drug-delivery system in which oxidized single-walled carbon nanohorns (oxSWNHs) were immobilized on a titanium (Ti) surface using material-binding peptides to enable localized drug delivery. For this purpose, we utilized a bispecific peptidic aptamer comprising a core sequence of a Ti-binding peptide and a SWNH-binding peptide to immobilize oxSWNHs on Ti. Scanning electron microscopy was used to confirm the presence of oxSWNHs adsorbed onto the Ti surface, and a quartz crystal microbalance was used to evaluate the binding process during oxSWNH adsorption. The oxSWNHs-ornamented Ti substrate was nontoxic to cells and released biologically active dexamethasone over a sustained period. This oxSWNHs-immobilized system can be used to modify the surface of Ti in implants and be loaded with drugs that stimulate osteogenesis and bone regeneration.