Mechanisms of Bushen Zhuyun Decoction Regulating Pituitary Cell GnRH Receptor and Downstream cAMP-PKA Signaling Pathway in Treating Infertility Due to Luteal Phase Defect
Mechanisms of Bushen Zhuyun Decoction Regulating Pituitary Cell GnRH Receptor and Downstream cAMP-PKA Signaling Pathway in Treating Infertility Due to Luteal Phase Defect
复制标题
补肾助孕汤调节垂体细胞GnRH受体及下游cAMP-PKA信号通路治疗黄体期缺陷性不孕症的机制研究
DOI：
10.17582/journal.pjz/2019.51.2.667.674
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发表时间：
2019-04-01
影响因子：
0.6
通讯作者：
Ji, Jianjian
中科院分区：
文献类型：
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作者：
Liu, Bei;Hong, Yanli;Ji, Jianjian
为观察补肾助孕汤对大鼠垂体细胞（RFC）的影响，探讨补肾助孕汤治疗黄体功能不全（LPD）不孕症的作用机制，采用西曲瑞克（Cetrorelix）阻断剂建立垂体细胞GnRHR拮抗模型。用含BSZYD的脑脊液（CSF）处理细胞。因此，共建立5组细胞，包括空白（+）组、西曲瑞克（+）组、CSF + BSZY（+）组、模型处理（+）组和相应的阻断剂处理组。检测各组培养上清中相关激素及其mRNA的分泌水平。同时检测RPC细胞中GnRHR、cAMP-PKA信号通路下游的枢纽基因和关键转录因子的mRNA和蛋白水平。与空白组相比，西曲瑞克组FSH、LH的分泌和转录水平明显降低，GnRHR的表达明显增加（p < 0.05）。
To observe the effect of Bushen Zhuyun Decoction (BSZYD) on rat pituitary cells (RFC), and to investigate the mechanism of BSZYD in treating infertility due to luteal phase defect (LPD), a GnRH-receptor (GnRHR) antagonized model of pituitary cells was established by using the blocking agent of Cetrorelix. The cells were treated with BSZYD-containing cerebrospinal fluid (CSF). Thus, a total of five groups of cells, including blank (+) group, Cetrorelix (+) group, CSF with BSZY (+) group, model-treated (+) group and corresponding blocker-treated group were established. Secretion levels of related hormone and their mRNAs were detected in the supernatant of each group. The mRNA and protein levels of GnRHR, the hub genes and key transcription factors in the downstream cAMP-PKA signaling pathways in RPC cells were also detected. The secretion and transcription levels of FSH and LH in Cetrorelix group were significantly decreased, while the expression of GnRHR was significantly increased compared to the blank group (p