Brain lactate uptake increases at the site of impact after traumatic brain injury
Brain lactate uptake increases at the site of impact after traumatic brain injury
复制标题
DOI：
10.1016/s0006-8993(00)01992-2
复制
发表时间：
2000-04-10
期刊：
影响因子：
2.9
通讯作者：
Bullock, R
中科院分区：
文献类型：
--
作者：
Chen, T;Qian, YZ;Bullock, R
虽然葡萄糖是正常大脑的主要碳水化合物能量底物，但过去10年发表的几项研究现在挑战了这一假设。激活的大脑增加其代谢，以满足受伤后糖酵解增加的能量需求。体外研究表明，乳酸盐本身可以作为维持突触功能的能量来源。在这项研究中，我们使用C-14-乳酸盐来测试的假设，即血乳酸是急性采取了受伤的大脑，液压冲击损伤（FPI）后，在大鼠。在FPI后立即静脉注射50 μ Ci放射性乳酸盐至损伤和假手术大鼠。30分钟后，取出脑，冷冻，并切成20 μ m的切片用于放射自显影。C-14-标记的摄取主要集中在损伤部位（大2.5倍），尽管未损伤的大脑也摄取了C-14-标记。损伤部位放射性乳酸盐浓度的增加表明，受损的大脑可能使用乳酸盐作为能量来源。(C)2000 Elsevier Science B. V.保留所有权利。
Although glucose is the main carbohydrate energy substrate for the normal brain, several studies published over the last 10 years now challenge this assumption. The activated brain increases its metabolism to meet increased energy demands by glycolysis after injury. In vitro studies now show that lactate alone can serve as an energy source to maintain synaptic function. In this study, we used C-14-lactate to test the hypothesis that blood lactate is acutely taken up by the injured brain, after fluid percussion injury (FPI) in the rat. 50 mu Ci radioactive lactate was injected i.v. immediately after FPI, in injured and sham rats. After 30 min, the brain was removed, frozen, and cut into 20 mu m sections for autoradiography. Uptake of C-14-label was mainly concentrated at the injury site (2.5 times greater) although uninjured brain also took up the C-14-label. This increased concentration of radioactive lactate at the injury site suggests that the injured brain may use the lactate as an energy source. (C) 2000 Elsevier Science B.V. All rights reserved.