Identification and mapping of a QTL (qDTY1.1) with a consistent effect on grain yield under drought
Identification and mapping of a QTL (qDTY1.1) with a consistent effect on grain yield under drought
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DOI：
10.1016/j.fcr.2012.02.028
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发表时间：
2012-05-13
影响因子：
5.8
通讯作者：
Kumar, Arvind
中科院分区：
文献类型：
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作者：
Ghimire, Krishna Hari;Quiatchon, Lenie A.;Kumar, Arvind
干旱胁迫下水稻产量QTL的定位和导入是提高水稻品种耐旱性的首选育种策略。Swarna和11264是两个高产水稻品种，广泛种植在南亚和东南亚的干旱地区，但它们对干旱高度敏感。Dhagaddeshi，一个传统的耐旱供体，与Swarna和IR 64杂交，开发两个重组自交系群体。在DS2010和DS2011期间，对两个群体在生殖阶段干旱胁迫和非胁迫下的籽粒产量进行表型分析。随后进行批量分离分析以确定与干旱下谷物产量相关的基因座。在两个群体的第1染色体RM431和RM12091之间均检测到一个干旱胁迫下籽粒产量的主效QTL qDTY（1.1）。在Dhagaddeshi × Swarna和Dhagaddeshi × IR 64群体中，qDTY（1.1）等位基因对干旱胁迫下的产量增加有贡献，分别解释了32.0%和9.3%的表型方差和24.9%和8.6%的加性效应。qDTY（1.1）的一致效应也在之前报道过。qDTY（1.1）在几个传统耐旱供体中的存在及其在遗传背景中的一致效应使其成为用于标记辅助育种以提高干旱敏感水稻品种的籽粒产量的合适QTL。(C)2012爱思唯尔有限公司版权所有。
The identification and introgression of QTLs for grain yield under drought is a preferred breeding strategy to improve the drought tolerance of popular elite rice varieties. Swarna and 11264 are two high-yielding rice varieties widely grown in rainfed areas of South and Southeast Asia but they are highly sensitive to drought. Dhagaddeshi, a traditional drought-tolerant donor, was crossed with Swarna and IR64 to develop two recombinant inbred line populations. The two populations were phenotyped for grain yield under reproductive-stage drought stress and non-stress during DS2010 and DS2011. Bulk segregant analysis was followed to identify the loci linked to grain yield under drought. A major-effect QTL, qDTY(1.1), for grain yield under drought was identified on chromosome 1 between the marker intervals RM431 and RM12091 in both populations. The Dhagaddeshi allele at qDTY(1.1) contributed to increased yield under drought and explained 32.0% and 9.3% of the phenotypic variance and 24.9% and 8.6% additive effect of the trial mean yield in Dhagaddeshi x Swarna and Dhagaddeshi x IR64 populations, respectively. A consistent effect of qDTY(1.1) has also been reported earlier. The presence of qDTY(1.1) in several traditional drought-tolerant donors and its consistent effect across the genetic backgrounds makes it a suitable QTL for use in marker-assisted breeding to improve the grain yield of drought-susceptible rice varieties. (C) 2012 Elsevier B.V. All rights reserved.