Macrophage migration inhibitory factor plays a critical role in mediating protection against the helminth parasite Taenia crassiceps.
Macrophage migration inhibitory factor plays a critical role in mediating protection against the helminth parasite Taenia crassiceps.
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巨噬细胞迁移抑制因子在介导针对蠕虫寄生虫青绦虫的保护中发挥着关键作用。
DOI：
10.1128/iai.71.3.1247-1254.2003
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发表时间：
2003
影响因子：
3.1
通讯作者：
Terrazas,LuisI
中科院分区：
文献类型：
--
作者：
Rodríguez-Sosa,Miriam;Rosas,LuciaE;David,JohnR;Bojalil,Rafael;Satoskar,AbhayR;Terrazas,LuisI
To determine the role of endogenous migration inhibitory factor (MIF) in regulation of immune response during murine cysticercosis caused by the helminth parasiteTaenia crassiceps, we analyzed the course ofT. crassicepsinfection in MIF−/−BALB/c mice. MIF−/−mice were highly susceptible toT. crassicepsand developed significantly higher parasite loads compared to similarly infected MIF+/+mice. Throughout the course of infection,Taenia crassicepssoluble antigen-stimulated spleen cells from both MIF+/+and MIF−/−mice produced significant and comparable levels of interleukin-4 (IL-4), but those from MIF−/−mice produced significantly more IL-13, as well as gamma interferon (IFN-γ), suggesting that the susceptibility of MIF−/−mice toT. crassicepswas not due to the lack of IFN-γ production. Interestingly, low levels of both total and specific immunoglobulin G2a were observed in MIF−/−cysticercotic mice despite the high IFN-γ levels; in addition, peritoneal macrophages obtained fromT. crassiceps-infected MIF−/−mice at different time points failed to respond efficiently to stimulation in vitro with lipopolysaccharide plus IFN-γ and produced significantly lower levels of IL-12, tumor necrosis factor alpha, and NO compared to those from MIF+/+mice. These findings demonstrate that MIF plays a critical role in mediating protection againstT. crassicepsin vivo. Moreover, these findings also suggest that impaired macrophage function rather than the lack of Th1 development may be responsible for mediating susceptibility toT. crassiceps.
